Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb #2717

No. Size Price
2717S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2717T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
2717 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 70, 72 Rabbit IgG
F 1:1600
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Rat, Hamster, Chicken, Bovine, Dog, Pig, Horse,

Specificity / Sensitivity

Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb detects endogenous levels of Zap-70 only when phosphorylated at Tyr319. It cross-reacts with endogenous levels of Syk when phosphorylated at Tyr352.

Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb只能检测内源的在Tyr319位点磷酸化的Zap-70蛋白。此抗体与在Tyr352位点磷酸化的Syk蛋白交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70.

此单克隆抗体是通过合成人源对应的Zap-70 Tyr319位点周围的磷肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells treated with hydrogen peroxide (2mM for 2 minutes) or with lambda phosphatase and extracts from Ramos cells treated with anti-human IgM (12 micrograms/ml for 2 minutes) using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb.Western免疫印迹。用Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb研究经过氧化氢(2mM, 2 min)或 lambda磷酸酶处理的Jurkat细胞的细胞抽提液和经anti-human IgM (12 micrograms/ml,2 min)处理的Ramos细胞的细胞提取液

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Ramos cells, untreated (blue) or treated with anti-IgM (green), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8885 was used as a secondary antibody.



Confocal immunofluorescent analysis of Ramos cells, human IgM treated (left) or untreated (right), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.


The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).

Syk 家族酪氨酸激酶Zap-70 在T 细胞和 NK 细胞中表达并在响应T细胞受体(TCR)而介导T细胞激活中发挥了重要的作用(1)。在TCR 招募下, Zap-70 通过自磷酸化和Src家族酪氨酸激酶Lck转磷酸化作用下快速的磷酸化几个酪氨酸残基(2-6)。酪氨酸的磷酸化与Zap-70激酶活性的增加和下游信号传导有关系。Zap-70 的表达与疾病的进程和慢性淋巴白血病患者的生存率有关(7,8)。

  1. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
  2. Iwashima, M. et al. (1994) Science 263, 1136-9.
  3. Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.
  4. Chan, A.C. et al. (1995) EMBO J 14, 2499-508.
  5. Williams, B.L. et al. (1999) EMBO J 18, 1832-44.
  6. Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.
  7. Wiestner, A. et al. (2003) Blood 101, 4944-51.
  8. Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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