Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb #2710

Spleen tyrosine kinase   Syk  

No. Size Price
2710S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2710T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
2710 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 72 Rabbit IgG
IP 1:50
F 1:400
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb detects endogenous levels of Syk protein only when phosphorylated at Tyr525/526 of human Syk or Tyr519/520 of mouse Syk. It also detects Syk protein when singly phosphorylated at Tyr526 of human Syk or Tyr520 of mouse Syk. It does not cross-react with other tyrosine-phosphorylated protein tyrosine kinases.

Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb兔单抗只能检测内源的在Tyr525/526位点磷酸化的人源Syk蛋白或Tyr519/520 位点磷酸化的鼠源Syk蛋白。此抗体也能检测在Tyr526位点磷酸化的人源Syk蛋白或Tyr520位点磷酸化的鼠源Syk蛋白。此抗体不与其它的酪氨酸残基磷酸化的酪氨酸激酶交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr525/526 of human Syk.

此单克隆抗体是通过合成人源对应的Syk Tyr525/526位点周围的磷肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos cells, untreated or treated with anti-IgM, using Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (upper) or Syk Antibody #2712 (lower).Western免疫印迹。用Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (上图) 或者Syk Antibody #2712 (下图)研究未经处理的和经anti-IgM处理的Ramos细胞的细胞提取液。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Ramos cells, untreated (blue) or treated with anti-IgM (green), using Phospho-Syk(Tyr525/526) (C87C1) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8885 was used as a secondary antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of Ramos cells, serum-starved (overnight; left) or IgM-treated (12 ug/ml, 2 minutes; right), using Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells, and Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation of Tyr323 provides a direct binding site to the TKB domain of Cbl (6,7). Tyrosine 352 of Syk is involved in the association of PLC-γ1 (8). Tyrosines 525 and 526 are located in the activation loop of the Syk kinase domain, and phosphorylation of Tyr525/526 of human Syk (equivalent to the Tyr519/520 of mouse Syk) is essential for Syk function (9).

Syk作为酪氨酸蛋白激酶在造血细胞的胞内信号转导中发挥重要作用(1-3)。 Syk能够与免疫受体的酪氨酸介导的活性区域(ITAMs)中的胞质部分结合(4)。它能偶联激活了的免疫受体进行下游的信号转导,而引起一系列的细胞应答,如细胞增殖、分化以及吞噬(4)。有证据显示Syk在无免疫反应的细胞中也有作用,Syk在人类的乳腺癌中是个潜在的肿瘤抑制因子(5)。 Syk蛋白的SH2-激酶结合区域中Tyr323位点是个磷酸化负调控位点。磷酸化的Tyr323为 Cbl的TKB区域提供了结合位点(6,7)。Syk的Tyr352位点参与了与 PLC-γ1 的结合 (8)。 Tyr525位点和526位点位于Syk的激酶区域的活性环上。磷酸化人源的Syk的Tyr525/526位点(等同于小鼠 Syk的 Tyr519/520位点) 对 Syk 的功能有重要的影响(9)。

  1. Cheng, A.M. and Chan, A.C. (1997) Curr Opin Immunol 9, 528-33.
  2. Kurosaki, T. (1997) Curr Opin Immunol 9, 309-18.
  3. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
  4. Turner, M. et al. (2000) Immunol Today 21, 148-54.
  5. Coopman, P.J. et al. (2000) Nature 406, 742-7.
  6. Deckert, M. et al. (1998) J Biol Chem 273, 8867-74.
  7. Rao, N. et al. (2001) EMBO J 20, 7085-95.
  8. Law, C.L. et al. (1996) Mol Cell Biol 16, 1305-15.
  9. Zhang, J. et al. (2000) J Biol Chem 275, 35442-7.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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