Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody #2701

Spleen tyrosine kinase   Syk   Syk-related tyrosine kinase   Zap   Zap70  

No. Size Price
2701L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
2701S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2701T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
2701 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 70 Zap-70, 72 Syk Rabbit
F 1:50
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Hamster, Monkey, Chicken, Bovine, Dog, Pig, Horse,

Specificity / Sensitivity

Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyr319. It cross-reacts with endogenous levels of Syk when phosphorylated at Tyr352.

Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody只能检测内源的在Tyr319位点磷酸化的Zap-70蛋白。此抗体与在Tyr352位点磷酸化的Syk蛋白交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆抗体是通过合成人源对应的Zap-70 Tyr319位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, starved for 16 hours, and treated with 2 mM H2O2 or with calf intestinal alkaline phosphatase (CIP), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody (upper) or control Zap-70 Antibody #2702 (lower).Western免疫印迹。用Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody (上图) 或者 control Zap-70 Antibody #2702 (下图)研究经16小时饥饿并用2 mM H2O2或者牛肠内碱性磷酸酶 (CIP)处理的Jurkat细胞的细胞提取液。

Flow Cytometry

Flow Cytometry

Two-color flow cytometric analysis of Jurkat cells, untreated (left) or anti-CD3 activated (right), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody and Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) mAb #9106. Anti-CD3 activation increases the intensity of label with both antibodies.双色通道流式细胞仪研究未经处理(左图)或经CD3 激活(右图)的Jurkat细胞,所用抗体为Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody 和 Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) mAb #9106.Anti-CD3激活能激活这两个抗体的标记效率。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or CD3 treated (green), using Phospho-Zap70 (Tyr319)/Syk (Tyr352) Antibody compared to a nonspecific negative control antibody (red).流式细胞仪研究未经处理的(蓝色)或经CD3处理的Jurkat细胞(绿色)。所用抗体为Phospho-Zap70 (Tyr319)/Syk (Tyr352) Antibody,非特异性的抗体为阴性对照。

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos cells, untreated or treated with anti-IgM (12 µg/ ml for 2 minutes), hydrogen peroxide (10 mM for 2 minutes) or lambda phosphatase, using Phospho-Zap-70 (Tyr319)/ Syk (Tyr352) Antibody.Western免疫印迹。用Phospho-Zap-70 (Tyr319)/ Syk (Tyr352) Antibody研究未经处理的和经anti-IgM (12 µg/ ml ,2 min), 过氧化氢 (10 mM,2 min)或 lambda 磷酸酶处理的Ramos细胞的细胞提取液。

IF-IC

IF-IC

Immunofluorescent analysis of Jurkat cells, CD3-treated (left) or untreated (right), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).免疫荧光分析CD3处理的(左图) 或未经处理(右图)的Jurkat细胞的细胞提取液,所用抗体为Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody (红色) Blue pseudocolor = DRAQ5™ (DNA 荧光染料)。

Background

The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).

Syk 家族酪氨酸激酶Zap-70 在T 细胞和 NK 细胞中表达并在响应T细胞受体(TCR)而介导T细胞激活中发挥了重要的作用(1)。在TCR 招募下, Zap-70 通过自磷酸化和Src家族酪氨酸激酶Lck转磷酸化作用下快速的磷酸化几个酪氨酸残基(2-6)。酪氨酸的磷酸化与Zap-70激酶活性的增加和下游信号传导有关系。Zap-70 的表达与疾病的进程和慢性淋巴白血病患者的生存率有关(7,8)。

Phosphorylation of Tyr319 is required for the assembly of a Zap-70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells (5,6). The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1 (9).

Tyr319位点的磷酸化对激活PLC-gamma1依赖和Ras依赖在配体引起的T细胞的级联信号反应中形成包含Zap-70信号复合体是必需的(5,6)。Syk的同源基因的Tyr352位点与PLC-gamma1也有关联(9)。

  1. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
  2. Iwashima, M. et al. (1994) Science 263, 1136-9.
  3. Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.
  4. Chan, A.C. et al. (1995) EMBO J 14, 2499-508.
  5. Williams, B.L. et al. (1999) EMBO J 18, 1832-44.
  6. Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.
  7. Wiestner, A. et al. (2003) Blood 101, 4944-51.
  8. Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.
  9. Law, C. L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.

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