Cell Signaling Technology

Product Pathways - NF-kB Signaling

Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697

IKK   ikk alpha   ikk beta   ikk-alpha   ikk-beta   ikkalpha   ikkbeta  

No. Size Price
2697L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
2697S 100 µl ( 10 western blots ) ¥3,780.00 现货查询 购买询价
2697T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
2697 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 85 IKK-alpha 87 IKK-beta Rabbit IgG
IHC-P 1:150
F 1:3200
IHC-F 1:150

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IHC-F=Immunohistochemistry (Frozen),

Homology

Species predicted to react based on 100% sequence homology: Bovine,

Specificity / Sensitivity

Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb detects IKKα only when phosphorylated at Ser176/180 and IKKβ only when phosphorylated at Ser177/181.

Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb只能检测内源的在Ser176/180位点磷酸化的IKKα蛋白和在Ser177/181位点磷酸化的IKKβ。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser176/180 of human IKKα.

此单克隆抗体是通过合成人源对应的IKKα Ser176/180位点周围的肽段来免疫动物而获得。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb.免疫组织化学染色分析石蜡包埋人结肠癌组织,图中所示为细胞质的定位。所用抗体为Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb in the presence of control peptide (left) or Phospho-IKK-alpha/beta (Ser176/180) Blocking Peptide #1023 (right).免疫组织化学染色分析石蜡包埋人乳腺癌组织。所用抗体为Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb, 在对照多肽 (左图) 或Phospho-IKK-alpha/beta (Ser176/180) 封闭多肽#1023 (有图)存在下。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb.免疫组织化学染色分析石蜡包埋未经处理 (左图) 和经λ phosphatase处理 (右图) 人结肠癌组织。所用抗体为Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human gall bladder (chronic cholecystitis), using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb.免疫组织化学染色分析石蜡包埋人胆囊(慢性胆囊炎)组织。所用抗体为Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung (chronic bronchitis), using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb.免疫组织化学染色分析石蜡包埋人慢性支气管炎组织。所用抗体为Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from TNF-alpha and Calyculin A treated HeLa and NIH/3T3 cells, using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb.Western免疫印迹。用Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb 研究经TNF-alpha 和 Calyculin A处理的HeLa 和 NIH/3T3 细胞的细胞提取液。

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen H1650 xenograft, showing cytoplasmic localization using Phospho-IKKα/β (Ser176/180)(16A6) Rabbit mAb.免疫组织化学染色研究冰冻H1650 异种移植物,图片显示的是细胞质的定位,所用的抗体为Phospho-IKKα/β (Ser176/180)(16A6) Rabbit mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb.Western免疫印迹。用Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb研究用TPA #9905, 80 nM 分化24小时后经1 μg/ml LPS 处理一定时间的 THP-1细胞的细胞提取液。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of THP-1 cells, untreated (blue) and with TPA and LPS (green) using IKK-α (Ser176/Ser180) phosphate Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8885 was used as a secondary antibody.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation; Ser177 and Ser181 in the activation loop of IKKβ (serine 176 and 180 in IKKα) are the specific sites whose phosphorylation causes conformational changes resulting in kinase activation (10-13).

NF-κB/Rel转录调控因子在细胞质中与IκB抑制蛋白结合以非活性形式存在(1-3)。大部分激活因素采用通常信号通路即磷酸化诱导并通过蛋白水解酶体介导降解IκB的方法激活NF-κB (3-7)。此通路中最重要的步骤是激活高分子量的IκB激酶(IKK)复合体,此复合体催化的反应中有三个紧密相关的IKK亚基参与完成,IKKα and IKKβ 作为激酶的催化单元,IKKγ作为调控单元(8,9)。IKK的激活依赖于特定位点的磷酸化:IKKβ活性环中的 Ser177和Ser181位点以及IKKα中的serine 176 和 180 位点,这些位点的磷酸化引起蛋白构象的变化从而激活激酶(10-13)。

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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