Cell Signaling Technology

Product Pathways - NF-kB Signaling

Phospho-IKKα/β (Ser176/180) Antibody II #2694

ikk-α   sc-21660  

No. Size Price
2694S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2694T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
2694 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 85 IKK-alpha 87 IKK-beta Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-IKKα/β (Ser176/180) Antibody II detects endogenous levels of IKKα and IKKβ only when phosphorylated at Ser176 and Ser180 or Ser177 and Ser181, respectively.

Phospho-IKKα/β (Ser176/180) Antibody II 分别能检测内源的在Ser176和Ser180位点磷酸化的IKKα蛋白和在Ser177和Ser181位点磷酸化的IKKβ。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a phosphopeptide corresponding to a region surrounding Ser177/181 of IKKβ. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是通过合成对应的IKKβ Ser177/181 位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, treated with TNF-α for the indicated times, using Phospho-IKKα/β (Ser176/180) Antibody II (upper) or IKKβ Antibody #2684 (lower).Western免疫印迹。用Phospho-IKKα/β (Ser176/180) Antibody II (上图) 或 IKKβ Antibody #2684 (下图)研究经TNF-α处理一定时间的HeLa细胞的细胞提取液。

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation; Ser177 and Ser181 in the activation loop of IKKβ (serine 176 and 180 in IKKα) are the specific sites whose phosphorylation causes conformational changes resulting in kinase activation (10-13).

NF-κB/Rel转录调控因子在细胞质中与IκB抑制蛋白结合以非活性形式存在(1-3)。大部分激活因素采用通常信号通路即磷酸化诱导并通过蛋白水解酶体介导降解IκB的方法激活NF-κB (3-7)。此通路中最重要的步骤是激活高分子量的IκB激酶(IKK)复合体,此复合体催化的反应中有三个紧密相关的IKK亚基参与完成,IKKα and IKKβ 作为激酶的催化单元,IKKγ作为调控单元(8,9)。IKK的激活依赖于特定位点的磷酸化:IKKβ活性环中的 Ser177和Ser181位点以及IKKα中的serine 176 和 180 位点,这些位点的磷酸化引起蛋白构象的变化从而激活激酶(10-13)。

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Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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