Cell Signaling Technology

Product Pathways - DNA Damage

Phospho-Chk2 (Thr68) Antibody #2661

checkpoint   chk  

No. Size Price
2661L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
2661S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2661T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
2661 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 62 Rabbit
IP 1:100
F 1:800
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Chk2 (Thr68) Antibody detects endogenous levels of Chk2 only when phosphorylated at threonine 68. The antibody does not cross-react with Chk2 phosphorylated at other sites. Phospho-Chk2 (Thr68) Antibody能够检测内源性苏氨酸(68位)的Chk2蛋白。该抗体不与其他位点磷酸化的Chk2蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr68 of human Chk2. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对Chk2蛋白苏氨酸(68位)的磷酸化肽段免疫动物,采用蛋白A和多肽亲和层析技术纯化生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or UV-treated (100 mJ/cm2, 1 hr recovery), using Phospho-Chk2 (Thr68) Antibody Western blot方法检测未处理和紫外处理(100 mJ/cm2, 恢复1小时)的HeLa细胞, 使用的抗体为 Phospho-Chk2 (Thr68) Antibody。

Western Blotting

Western Blotting

Western blot analysis of extracts from Cos cells, untreated or UV-treated (100 mJ/cm2, 1 hr recovery), using Phospho-Chk2 (Thr68) Antibody Western blot方法检测未处理和紫外处理(100 mJ/cm2, 恢复1小时)的Cos细胞, 使用的抗体为 Phospho-Chk2 (Thr68) Antibody。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or UV-treated (right), using Phospho-Chk2 (Thr68) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光法检测HeLa细胞,未处理(左图)或紫外处理组(右图),使用的抗体为Phospho-Chk2 (Thr68) Antibody ,呈绿色。肌动蛋白纤维用DY-554鬼笔环肽标记,呈红色。蓝色伪彩为DNA荧光染料(产品信息为DRAQ5® #4084 )。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, untreated (blue) and UV-treated (green), using Phospho-Chk2 (Thr68) Antibody. 流式细胞术检测未处理(蓝色)和紫外处理的(绿色)HeLa细胞,使用的抗体为 Phospho-Chk2 (Thr68) Antibody。

Background

Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain (8). Chk2是哺乳动物中和芽殖酵母Rad53和裂殖酵母Cds1检验点激酶同源的蛋白(1-3)。Chk2的氨基末端结构域包含有一个七丝氨酸或者苏氨酸残基(Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68)组成的系列,每个系列紧跟着谷氨酸(SQ 或TQ模体)。这些地方是已知的ATM/ATR激酶磷酸化的首选作用位点(4,5)。电离辐射(IR)引起DNA损伤之后,UV照射处理或者羟基脲处理,这个区域的68位苏氨酸和其他位点被ATM/ATR磷酸化(5-7)。因此,SQ/TQ簇结构域似乎具有调节功能。第68位苏氨酸的磷酸化是后续激活步骤的先决条件,激活归因于激酶结构域激活回路中Chk2第383位和387位苏氨酸残基的自体磷酸化(8)。

  1. Allen, J.B. et al. (1994) Genes Dev. 8, 2401-2415.
  2. Weinert, T.A. et al. (1994) Genes Dev. 8, 652-665.
  3. Murakami, H. and Okayama, H. (1995) Nature 374, 817-819.
  4. Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186.
  5. Matsuoka, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 10389-10394.
  6. Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765.
  7. Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934-5936.
  8. Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications