Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat4 (C46B10) Rabbit mAb #2653

Stat   Stat-4  

No. Size Price
2653S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2653 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 81 Rabbit
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, ChIP=Chromatin IP,

Specificity / Sensitivity

Stat4 (C46B10) Rabbit mAb detects endogenous levels of total Stat4 protein.

Stat4 (C46B10) Rabbit mAb 能够检测内源Stat4蛋白总体水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys151 of Stat4.

此单克隆抗体是通过合成对应的Stat4 Lys151位点周围的肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from ACHN, SR and Caki cell lines and from rat thymus using Stat4 (C46B10) Rabbit mAb.Western免疫印迹。用Stat4 (C46B10) Rabbit mAb研究ACHN, SR 和Caki 细胞系的细胞提取液以及大鼠胸腺提取液。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NK-92 cells starved of IL-2 overnight then treated with IL-12 (10ng/ml) for 4h and either 20 μl of Stat4 (C46B10) Rabbit mAb #2653 or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, human PRF1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。NK-92细胞培养至4 x 106,IL-2饥饿处理过夜后经IL-12(10ng/ml)诱导4小时,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用20 μlStat4 (C46B10) Rabbit mAb #2653抗体或2μl Normal Rabbit IgG #2729 。用human IRF-1 promoter primers, human PRF1 promoter primers和SimpleChIP® Human α Satellite Repeat Primers #4486对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and from rat thymus using Stat4 (C46B10) Rabbit mAb.

Background

The Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins resulting in their nuclear translocation, binding to specific DNA elements and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8).

Jak-Stat信号通路被很多的细胞因子,生长因子和激素所利用(1)。受体诱导的 Jak家族蛋白的酪氨酸磷酸化引起Stat蛋白的磷酸化导致他们入核,与DNA原件结合并活化转录。Stats所调控反应显著的专一性和范围一部分受控于组织特异性表达不同的细胞因子受体,Jaks 和 Stats同时也与不同的Stat成员偶联到不同的受体上的组合有关(2)。Stat4主要是在脾, 胸腺和睾丸组织中表达,并且作为IL-12的调节蛋白而得到研究(3-8)。

  1. Darnell, J.E. et al. (1994) Science 264, 1415-1421.
  2. Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
  3. Zhong, Z. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 4806-4810.
  4. Yamamoto, K. et al. (1994) Mol. Cell Biol. 14, 4342-4349.
  5. Jacobson, N.G. et al. (1995) J. Exp. Med. 181, 1755-1762.
  6. Bacon, C.M. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 7307-7311.
  7. Thierfelder, W.E. et al. (1996) Nature 382, 171-174.
  8. Kaplan, M.H. et al. (1996) Nature 382, 174-177.
  9. Visconti, R. et al. (2000) Blood 96, 1844-52.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications