Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-PRAS40 (Thr246) Antibody #2640

40 kDa AKT substrate   40 kDa AKT substrate 1   40 kDa proline-rich AKT substrate 1   AKT1 substrate   AKT1 substrate 1   AKT1S   AKT1S1   AKTS   AKTS1   pras   pras40   Proline-rich AKT1 substrate   Proline-rich AKT1 substrate 1  

No. Size Price
2640S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2640 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 40 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-PRAS40 (Thr246) Antibody detects endogenous levels of PRAS40 protein only when phosphorylated at Thr246.

Phospho-PRAS40 (Thr246) Antibody检测内源性的Thr246位点磷酸化的PRAS40蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Thr246 of human PRAS40. Antibodies are purified by peptide affinity chromatography.

该多克隆抗体是由合成的人源的针对PRAS40 蛋白Thr246位点磷酸化肽段免疫动物而生产的。抗体由肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, serum-starved for 14 hours and then either left untreated or treated with insulin (150 nM) for 15 minutes, using Phospho-PRAS40 (Thr246) Antibody.

Western blot 方法检测细胞提取物:血清饥饿14小时的NIH/3T3细胞,然后未处理和insulin (150 nM)处理15分钟,使用的抗体是Phospho-PRAS40 (Thr246)Antibody。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, serum-starved for 14 hours and then either left untreated or treated with insulin (150 nM) for 15 minutes, using Phospho-PRAS40 (Thr246) Antibody. The experiment was performed in the presence of phospho-peptide specific to phospho-PRAS40 (Thr246) (upper panel), the corresponding nonphospho-peptide (middle panel) and in the absence of any blocking peptide (lower panel).

Western blot 方法检测细胞提取物:血清饥饿14小时的NIH/3T3细胞,然后未处理和insulin (150 nM)处理15分钟,使用的抗体是Phospho-PRAS40 (Thr246)Antibody。上图是使用特异性识别phospho-PRAS40 (Thr246)的磷酸化肽段,中图是对应的非磷酸化肽段,下图是不用任何封闭肽。

Background

Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 protein (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5).

许多生长因子和激素诱导PI3K激酶信号通路,从而激活下游效应蛋白,例如丝氨酸/苏氨酸激酶Akt(1,2)。一个已知的Akt的底物是一个40 kDa的,富含脯氨酸的蛋白(PRAS40),可以结合14-3-3蛋白(2)。PRAS40还能够结合mTOR,传递Akt信号到mTOR复合物。抑制mTOR信号可以刺激PRAS40结合到mTOR上,反过来抑制mTOR的活性(3)。在缺失胰岛素的细胞内PRAS40和mTOR复合物1(mTORC1)中的Raptor 结合,进而抑制细胞周期蛋白Rheb调节的mTORC1信号通路的激活。Akt磷酸化PRAS40的Thr246位点,解除PRAS40对mTORC1的抑制(4)。反过来mTORC1磷酸化PRAS40的Ser183(5)。

  1. Cantley, L.C. (2002) Science 296, 1655-7.
  2. Kovacina, K.S. et al. (2003) J Biol Chem 278, 10189-94.
  3. Vander Haar, E. et al. (2007) Nat Cell Biol 9, 316-23.
  4. Sancak, Y. et al. (2007) Mol Cell 25, 903-15.
  5. Oshiro, N. et al. (2007) J Biol Chem 282, 20329-39.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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