Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

BTAF1 Antibody #2637

No. Size Price
2637S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2637 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 200 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine, Horse,

Specificity / Sensitivity

BTAF1 Antibody recognizes endogenous levels of total BTAF1 protein.

BTAF1 Antibody能够检测内源性BTAF1总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala220 of human BTAF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

通过合成的人源BTAF1蛋白Ala220位点周围相应的多肽去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using BTAF1 Antibody.

使用BTAF1 Antibody,免疫印迹(Western blot)分析不同细胞中BTAF1蛋白水平。

Background

B-TATA binding protein-associated factor 1 (BTAF1) is a human homolog of the yeast protein modifier of transcription 1 (MOT1) (1). BTAF1 negatively regulates transcription through interaction with TATA binding protein (TBP), an important component of the B-TFIID complex (2,3). BTAF1 is a member of the SNF2 family of ATPases that utilizes ATP to remove TBP from B-TFIID, thereby preventing formation of the transcription pre-initiation complex (PIC) (3-5). BTAF1 actively removes TBP from DNA, which prevents non-specific TBP-DNA interactions and promotes disassembly of inactive forms of the PIC. This activity is thought to be critical for maintaining a pool of free TBP that is necessary for the assembly of the PIC during transcription activation (6,7).

B-TATA binding protein-associated factor 1 (BTAF1)是一个酵母蛋白modifier of transcription 1 (MOT1)的人源同系物(1)。BTAF1通过与TATA binding protein (TBP)相互作用负性调节转录,而TATA binding protein (TBP)是B-TFIID复合物的一个重要成分(2,3)。BTAF1是ATPases的SNF2家族成员,该激酶能利用ATP去从B-TFIID上移除TBP,因此抑制转录前起始复合物(PIC)的形成 (3-5)。BTAF1积极地移除DNA上的TBP,这抑制非特异性TBP-DNA相互作用,并且促进PIC的非活性形式的分解。这活性被认为对于维持一批游离的TBP是至关重要的,而游离的TBP对于在转录激活期间PIC的装配是必要的(6,7)。

  1. Chicca, J.J. et al. (1998) Mol Cell Biol 18, 1701-10.
  2. Pereira, L.A. et al. (2004) J Biol Chem 279, 21802-7.
  3. Auble, D.T. (2009) Trends Biochem Sci 34, 49-52.
  4. Auble, D.T. et al. (1994) Genes Dev 8, 1920-34.
  5. de Graaf, P. et al. (2010) J Cell Sci 123, 2663-71.
  6. Dasgupta, A. et al. (2005) EMBO J 24, 1717-29.
  7. Huisinga, K.L. and Pugh, B.F. (2007) Genome Biol 8, R46.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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