Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody #2611

PRK   PRK-1   PRK1   sc-12891  

No. Size Price
2611S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2611 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 120 Phospho-PRK1. 140 Phospho-PRK2. Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-PRK1 (Thr774)/ PRK2 (Thr816) Antibody detects endogenous levels of PRK1 and PRK2 only when phosphorylated at Thr774 or Thr816, respectively. This antibody also detects PKC zeta and lambda when phosphorylated at Thr 410 and 403, respectively.

Phospho-PRK1 (Thr774)/ PRK2 (Thr816)抗体识别内源性的Thr774磷酸化PRK1蛋白或Thr816磷酸化的PRK2蛋白。它也可以识别Thr410磷酸化的PKCζ和Thr403磷酸化的PKCλ。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr774 of human PRK1. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体用与人类PRK1中Thr774位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or calf intestinal alkaline phosphotase (CIP)-treated, and 293 cells, untreated or calyculin A-treated, using Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody (upper) or PRK1/PRK2 antibody (lower).

对Jurkat细胞(未处理,或小牛肠碱性磷酸酶(CIP)处理)和293细胞(未处理或calyculin A处理),使用Phospho-PRK1 (Thr774)/PRK2 (Thr816)抗体(上图)或PRK1/PRK2抗体(下图)进行Western blot分析。

Background

The protein kinase C-related kinases (PRKs) are a subfamily of Ser/Thr-specific kinases with a catalytic domain highly homologous to the PKC family (1-3). They are effectors of Rho family GTPases (4-6) and are activated by fatty acids and phospholipids in vitro (7,8). Activation in vitro and in vivo involves the activation loop phosphorylation of PRK1 (Thr774)/PRK2 (Thr816) by PDK1 (9,10).

蛋白激酶C相关激酶(PRKs)是一种丝氨酸/苏氨酸特异性激酶亚家族,具有与PKC家族具有高度同源性的催化结构域(1-3)。它们是Rho家族GTP酶的效应物(4-6),在体外由脂肪酸和磷脂激活(7,8)。体外和体内的激活涉及PDK1磷酸化修饰PRK1 (Thr774)/PRK2 (Thr816)的活性环(9,10)。

  1. Mukai, H. et al. (1994) Biochem. Biophys. Res. Commun. 199, 897-904.
  2. Morrice, N.A. et al. (1994) J. Biol. Chem. 269, 20040-20046.
  3. Palmer, R.H. et al. (1994) FEBS Lett. 356, 5-8.
  4. Watanabe, G. et al. (1996) Science 271, 645-648.
  5. Amano, M. et al. (1996) Science 271, 648-650.
  6. Vincent, S. and Settleman, J. (1997) Mol. Cell. Biol. 17, 2247-2256.
  7. Morrice, N.A. et al. (1994) FEBS Lett. 351, 171-175.
  8. Palmer, R.H. et al. (1995) J. Biol. Chem. 270, 22412-22416.
  9. Flynn, P. et al. (2000) J. Biol. Chem. 275, 11064-70.
  10. Dong, L.Q. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 5089-94.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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