Product Pathways - Metabolism
Myoglobin (D2F5X) Rabbit mAb #25919
|25919S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|25919||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-F=Immunofluorescence (Frozen),
Specificity / Sensitivity
Myoglobin (D2F5X) Rabbit mAb recognizes endogenous levels of total myoglobin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu50 of human myoglobin protein.
Confocal immunofluorescent analysis of mouse skeletal (left) or smooth (right) muscle using Myoglobin (D2F5X) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Myoglobin (MB) is an oxygen-binding protein that contains one polypeptide chain and one heme group. It is expressed in vertebrate skeletal and cardiac muscles where it plays an essential role in the storage and transport of oxygen to mitochondria. Reversible oxygen binding occurs by a linkage with the imidazole nitrogen of the 91st histidine residue in the myoglobin chain. Research studies indicate that the blockade of myoglobin in isolated cardiac myocytes mimics hypoxia when electrically stimulated for paced contractions (1). During fetal development, myoglobin is required to support cardiac function (2). Diving mammals are known to have high concentrations of myoglobin in their blood, which may contribute to their ability to endure long periods of oxygen deprivation during deep dives (3).
- White, R.L. and Wittenberg, B.A. (1993) Biophys J 65, 196-204.
- Meeson, A.P. et al. (2001) Circ Res 88, 713-20.
- Davis, R.W. (2014) J Comp Physiol B 184, 23-53.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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Tween is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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