Cell Signaling Technology

Product Pathways - Phosphatases

Phospho-PP1α (Thr320) Antibody #2581

Phosphatase   PP1-α   PPP1CA   prophos  

No. Size Price
2581S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2581 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 38 Rabbit
IHC-P 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Phospho-PP1α (Thr320) Antibody detects PP1α only when phosphorylated at Thr320. This antibody may cross-react with the phosphorylated δ or γ isoforms of PP1.Phospho-PP1α (Thr320) Antibody能够检测内源性苏氨酸(320位)磷酸化的PP1α蛋白。该抗体也许与其他位点磷酸化的PP1蛋白的磷酸化δ或γ亚基发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr320 of human PP1α. Antibodies are purified by protein A and peptide affinity chromatography.该多克隆抗体是由合成的人源的针对PP1α蛋白苏氨酸(320位)的磷酸化肽段免疫动物,采用蛋白A和多肽亲和层析技术纯化生产的。

Western Blotting

Western Blotting

Western blot analysis of Recombinant PP1α, incubated with or without cdc2 protein kinase in the presence of ATP and kinase assay buffer at 30ºC for 30 minutes, using Phospho-PP1α (Thr320) Antibody (upper) or control PP1α Antibody #2582 (lower).Western blot方法检测重组PP1α,在ATP和激酶分析液存在的情况下,分为非处理组和cdc2蛋白激酶处理组,于30ºC孵育30分钟,使用的抗体为Phospho-PP1α (Thr320) Antibody (上图) 或control PP1α Antibody #2582 (下图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, asynchronous (lane 1), G2/M (lane 2) or released from G2/M for 6 hours (lane 3), using Phospho-PP1α (Thr320) Antibody (upper) or PP1α Antibody #2582 (lower).Western blot 方法检测Hela细胞提取物,分为非同步化组(泳道1),G2/M停滞组(泳道2)和从G2/M释放6小时组(泳道3),使用的抗体为Phospho-PP1α (Thr320) Antibody (上图) 或PP1α Antibody #2582 (下图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Phospho-PP1α (Thr320) Antibody.免疫组织化学方法检测石蜡包埋人类乳腺癌组织,显示核定位,使用的抗体为 Phospho-PP1α (Thr320) Antibody.


Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (1). Involvement in cell cycle regulation is one of the biological functions of PP1 (1). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (2,3). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (4). 1型蛋白磷酸酶(PP1)是真核细胞中一种高度保守的丝氨酸/苏氨酸磷酸酶。PP1具有四个异构体:PP1α, PP1δ, PP1γ1 和PP1γ2 (1)。PP1的一个生物学功能是参与细胞周期调节(1)。有描述认为PP1在有丝分裂期能够去磷酸化Rb 和cdc25(2,3)。Cdc20介导的PP1α第320位苏氨酸细胞周期依赖性磷酸化能够抑制PP1α的活性(4)。

  1. Rubin, E. et al. (1998) Front Biosci 3, D1209-19.
  2. Durfee, T. et al. (1993) Genes Dev 7, 555-69.
  3. Izumi, T. et al. (1992) Mol Biol Cell 3, 927-39.
  4. Kwon, Y.G. et al. (1997) Proc Natl Acad Sci U S A 94, 2168-73.

Application References

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