Cell Signaling Technology

Product Pathways - Neuroscience

GIRK2 (D6C2T) Rabbit mAb #25797

No. Size Price
25797S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
25797 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 46 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

GIRK2 (D6C2T) Rabbit mAb recognizes endogenous levels of total GIRK2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg46 of human GIRK2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human GIRK2 protein (hGIRK2-Myc/DDK; +), and membrane extracts of human, mouse, and rat brain using GIRK2 (D6C2T) Rabbit mAb.



Immunoprecipitation of GIRK2 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is GIRK2 (D6C2T) Rabbit mAb. Western blot analysis was performed using GIRK2 (D6C2T) Rabbit mAb and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678.


GIRK2 is a member of G protein-coupled inwardly rectifying potassium channel family proteins (GIRKs). GIRK family proteins allow potassium to flow into the cell and therefore control cellular excitability in the central nervous system, heart, and pancreas (1-4). Activation of most GIRK channels requires heterologous subunit assembly and the presence of ATP (5-7). GIRK2 is abundantly expressed in the brain, where it is involved in pain perception. It is also required for peripheral opioid-mediated analgesia (8). Additionally GIRK2 localizes to pancreatic β cells and regulates insulin secretion (9,10). Mutations in the KCNJ6 gene encoding GIRK2 are associated with Keppen-Lubinsky Syndrome, a rare disease characterized by severe developmental delay, facial dysmorphism, and intellectual disability (11).

  1. Lesage, F. et al. (1995) J Biol Chem 270, 28660-7.
  2. Kubo, Y. et al. (1993) Nature 364, 802-6.
  3. Krapivinsky, G. et al. (1995) Nature 374, 135-41.
  4. Isomoto, S. et al. (1997) Jpn J Physiol 47, 11-39.
  5. Duprat, F. et al. (1995) Biochem Biophys Res Commun 212, 657-63.
  6. Corey, S. and Clapham, D.E. (1998) J Biol Chem 273, 27499-504.
  7. Han, J. et al. (2003) J Physiol 550, 693-706.
  8. Nockemann, D. et al. (2013) EMBO Mol Med 5, 1263-77.
  9. Ferrer, J. et al. (1995) J Biol Chem 270, 26086-91.
  10. Sakura, H. et al. (1995) FEBS Lett 367, 193-7.
  11. Masotti, A. et al. (2015) Am J Hum Genet 96, 295-300.

Application References

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