Cell Signaling Technology

Product Pathways - Development

TCF4 (C9B9) Rabbit mAb #2565

LEF   sc-13027   sc-8631   TCF-4   TCF4   TCF7L2  

No. Size Price
2565S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2565 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 58, 79 Rabbit IgG
IP 1:50
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

TCF4 (C9B9) Rabbit mAb detects endogenous levels of total TCF4 protein.

TCF4 (C9B9)Rabbit mAb兔单抗可以识别内源的总TCF4蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu81 of human TCF4.

此单抗由合成肽段免疫动物产生,该肽段与人TCF4蛋白的 Glu81氨基酸残基序列一致。

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from HCT116, DLD1 and HepG2 cells using TCF4 (C9B9) Rabbit mAb.

使用TCF4 (C9B9)Rabbit mAb兔单抗对HCT116,DLD1和HepG2细胞全细胞提取物进行western blot分析。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT 116 cells and either 10 μl of TCF4 (C9B9) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106 HCT 116细胞与10μl TCF4 (C9B9) Rabbit mAb 兔单抗或2μl Normal Rabbit IgG #2729进行染色质免疫共沉淀。富集到的DNA使用SimpleChIP®人CaMK2D Intron 3引物#5111,人c-Myc promoter引物和SimpleChIP®人α Satellite Repeat 引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量与input染色质总量对比进行相对定量,其中Input染色质量设定值为1。


LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5).

LEF1和TCF是高迁移率族蛋白(HMG)中DNA结合蛋白家族的成员中的一类转录因子,包括:Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1)。LEF1和TCF1最初被认为是调节早期淋巴发育的重要因子(2),并作为Wnt的下游信号发挥作用。LEF1和TCF结合Wnt反应元件为β-catenin提供停泊位点,β-catenin随后转移到细胞核促进Wnt信号通路靶基因的转录激活(3)。在包括结肠癌(4,5)在内的多种癌症发生相关的Wnt信号通路的发展和异常活化中,都会伴随着LEF1和TCF表达水平的动态变化。

LEF1 和TCF是转录因子高迁移组(HMG)DNA结合蛋白家族的成员,由淋巴增强因子1(LEF1),T细胞因子1(TCF1),TCF3和TCF4组成(1)。LEF1和TCF1最初发现是Wnt信号通路下游调控早期淋巴细胞发育的重要因子(2)。LEF1和TCF与Wnt反应元件结合,为β-catenin提供结合位点,以转移到细胞核中促进Wnt下游靶基因的转录(3)。LEF1和TCF蛋白在发育过程中表现活跃,异常激活的Wnt信号通路与包括结肠癌在内的多种癌症密切相关(4,5)。

TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting studies indicate that TCF4 is required to maintain the crypt stem cells of the small intestine (6,7). TCF4 has several splicing isoforms that are expressed differentially in tissues and during cancer progression (8,9). Studies also indicate that a variant of the TCF4 gene confers an increased risk of type 2 diabetes (10).

TCF4,也称TCF7L2,在发育进程中广泛表达。基因靶标研究显示TCF4是维持小肠的隐窝干细胞所必需的(6,7)。TCF4有一些剪切的异构体在组织和癌症进程中差异表达(8,9)。研究也显示TCF4的突变型可能会增加2型糖尿病的发生风险(10) 。

  1. Waterman, M.L. (2004) Cancer Metastasis Rev 23, 41-52.
  2. Schilham, M.W. and Clevers, H. (1998) Semin Immunol 10, 127-32.
  3. Brantjes, H. et al. (2002) Biol Chem 383, 255-61.
  4. Reya, T. and Clevers, H. (2005) Nature 434, 843-50.
  5. Logan, C.Y. and Nusse, R. (2004) Annu Rev Cell Dev Biol 20, 781-810.
  6. Cho, E.A. and Dressler, G.R. (1998) Mech. Dev. 77, 9-18.
  7. Korinek, V. et al. (1998) Nat. Genet. 19, 379-383.
  8. Howng, S.L. et al. (2004) Int. J. Oncol. 25, 1685-1692.
  9. Shiina, H. et al. (2003) Clin. Cancer Res. 9, 2121-2132.
  10. Grant, S.F. et al. (2006) Nat. Genet. 38, 320-323.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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