Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-CDK2 (Thr160) Antibody #2561

cdk   cyclin  

No. Size Price
2561S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2561 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 33 Rabbit
IP 1:50
F 1:25
E-P 1:2000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-CDK2 (Thr160) Antibody detects endogenous levels of CDK2 only when phosphorylated at threonine 160. The antibody weakly cross-reacts with cdc2 phosphorylated at Thr161. It does not cross-react with other phosphorylated cyclin dependent kinases. Phospho-CDK2 (Thr160) Antibody 能够检测内源性苏氨酸(160位)磷酸化的CDK2蛋白,该抗体与苏氨酸(161位)磷酸化的cdc2蛋白有轻微交叉反应,与其他磷酸化周期蛋白依赖性激酶不发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr160 of human CDK2. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对CDK2蛋白的苏氨酸(160位)磷酸化肽段免疫动物,利用蛋白A和多肽亲和层析技术生产的。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells, using Phospho-CDK2 (Thr160) Antibody versus propidium iodide (DNA content). The boxed population indicates phospho-CDK2 (Thr160)-positive cells. 流式细胞术分析未处理的Jurkat细胞,使用的抗体为Phospho-CDK2 (Thr160) Antibody ,PI染色法检测DNA含量。框内的细胞群为phospho-CDK2 (Thr160)阳性细胞。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells synchronized in G0, G1/S or Mitosis, using Phospho-CDK2 (Thr160) Antibody.


Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15 (1). Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1 (2). Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160 (3), which is mediated by CAK, a complex of CDK7 and cyclin H (4). CDK2/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2 (5). 周期蛋白依赖性激酶2(p33CDK2)是细胞周期机制的重要组成部分。类似于p34cdc2,激酶活性的调节经由磷酸化状态以及周期蛋白亚基和CDK抑制剂之间的关系。磷酸化抑制通常发生在14位和15位苏氨酸(1)。CDK2-周期蛋白复合体的抑制也与p27 Kip1和 p21 Waf1/Cip1之间的关系有关(2)。CDK2复合体的磷酸化需要cdc25磷酸酶和160位苏氨酸磷酸化引(由CDK7和cyclin H组成的CAK复合体介导)起的14位和15位苏氨酸去磷酸化。 CDK2/周期蛋白E激酶活性对于G1到S期的转换和Rb蛋白的磷酸化具有重要意义。在S期,活化的CDK2/周期蛋白A复合体支配和磷酸化E2F,而活化的CDK2复合体持续在G2期的胞核中存在(5)。

  1. Morgan, D.O. (1995) Nature 374, 131-4.
  2. Poon, R.Y. et al. (1996) J Biol Chem 271, 13283-91.
  3. Gu, Y. et al. (1992) EMBO J 11, 3995-4005.
  4. Fesquet, D. et al. (1993) EMBO J 12, 3111-21.
  5. Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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