Cell Signaling Technology

Product Pathways - Protein Stability

Calpain 1 Large Subunit (Mu-type) Antibody #2556

Calcium-activated-neutral-proteinase   Calpain-1-Large-[catalytic]-subunit   CAN1   CANP   CANPL1   CAPN1   EC   Micromolar-calpain   Mu-type   muCANP  

No. Size Price
2556S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2556 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 75, 80 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Calpain 1 Large Subunit (Mu-type) Antibody detects endogenous levels of total calpain 1 (large subunit) protein. The antibody detects full-length calpain 1 as well as calpain 1 autoproteolytically cleaved at leucine 28.

Calpain 1 Large Subunit (Mu-type)抗体可以识别内源性总的calpain1(大亚基)蛋白。抗体可以识别全长的钙蛋白酶1和在28位亮氨酸处自发进行了蛋白裂解的钙蛋白酶1。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human sequence of calpain 1 (large subunit). Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and U937 cells, using Calpain 1 Large Subunit (Mu-type) Antibody.使用Calpain 1 Large Subunit (Mu-type)抗体对Jurkat和U937细胞提取物进行western blot分析。


Calpain is a calcium-dependent thiol proteinase that is functionally active as a heterodimer composed of a small regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation. The regulation of calpain in vivo is the subject of many current studies, which suggest that proteolytic activity is regulated post-transcriptionally by mechanisms such as calcium requirements, subcellular localization of the heterodimer, phosphorylation via the EGFR-Erk signaling cascade, endogenous inhibitors (calpastatin) and autoproteolytic cleavage (1). Calpastatin negatively regulates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28 (2). Calpain influences cell migration by modifying rather than degrading its substrates responsible for cell adhesion and cytoskeletal arrangement. Control of calpain activity has caught the attention of drug development since limiting its activity could mute invasiveness of tumors or chronic inflammation (1).


  1. Perrin, B.J. and Huttenlocher, A. (2002) Int. J. Biochem. Cell Biol. 34, 722-725.
  2. Melloni, E. et al. (1996) Biochem. Biophys. Res. Commun. 229, 193-197.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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