Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-FGF Receptor 1 (Tyr766) (1E5) Rabbit mAb #2544


No. Size Price
2544S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2544 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 120, 145 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-FGF Receptor 1 (Tyr766) (1E5) Rabbit mAb detects transfected levels of FGFR-1 only when phosphorylated at tyrosine 766. The antibody may cross-react with other FGFR family members and some activated protein tyrosine kinases including EGFR and insulin/IGF-I receptors.

磷酸化FGF Receptor 1 (Tyr766) (1E5) 兔单抗仅在Tyr766被磷酸化后才能检测到转染表达的FGFR-1蛋白的存在。本抗体可能与FGFR 家族其它成员以及一些活化的酪氨酸激酶,包括EGFR 和 胰岛素/IGF-I receptors,有交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr766 of human FGF receptor-1.

单克隆抗体通过用多肽免疫动物得到,该磷酸化多肽是根据人的FGF receptor-1蛋白Tyr766附近的氨基酸序列合成的。

Western Blotting

Western Blotting

Western blot analysis of cell extracts from COS cells overexpressing human FGFR1 and control COS cells, using Phospho-FGF Receptor-1 (Tyr766) (1E5) Rabbit mAb. Before antibody probing, the transferred membrane was untreated (upper) or CIP treated (lower) to confirm the phospho-specificity of the antibody. Western blot法检测以下细胞提取物:高表达人FGFR1的COS细胞和对照COS细胞。使用抗体为:Phospho-FGF Receptor-1 (Tyr766) (1E5) Rabbit mAb。在抗体孵育前,为确定抗体的抗磷酸化蛋白特异性,转了蛋白的膜未经(上)或经过(下)CIP预处理。


Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3, and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5).

成纤维细胞生长因子 (FGFs)通过细胞表面的酪氨酸激酶受体传递信号,对靶细胞产生促有丝分裂和血管生成的效应。已知FGF受体家族有四个成员:FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3和FGFR-4。每个受体都有一个胞外配体结合区、跨膜区和一个胞内激酶区(1)。在结合配体和二聚化之后,受体的特定的酪氨酸位点发生磷酸化(2)。在FGFR-1的羧基端有7个酪氨酸位点能被磷酸化:Tyr463, 583, 585, 653, 654, 730和 766。Tyr653 和Tyr654对激活的FGFR的催化活性十分重要,对信号传导也是必要的(3)。其它的磷酸化酪氨酸位点能够为下游信号分子,如Crk 和PLCγ,提供结合位点(4,5)。

Autophosphorylation of Tyr766 of FGFR1 is critical for phospholipase C (PLC) binding and activation and also plays a role in the negative regulation of FGFR1 activity in vivo (6).

FGFR1的Tyr766位点自磷酸化对于磷脂酶 C (PLC) 的结合和活化,以及体内FGFR1活性的负调控均起着十分重要的作用(6).

  1. Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97.
  2. Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8.
  3. Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89.
  4. Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78.
  5. Larsson, H. et al. (1999) J Biol Chem 274, 25726-34.
  6. Partanen, J. et al. (1998) Genes Dev 12, 2332-44.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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