Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-cdc2 (Thr14) Antibody #2543

CDC   CDC28   CDK1  

No. Size Price
2543S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2543 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Hamster,Monkey, Endogenous 34 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, D. melanogaster, Xenopus, Bovine,

Specificity / Sensitivity

Phospho-cdc2 (Thr14) Antibody detects endogenous levels of cdc2 (CDK1) only when phosphorylated at Thr14. Based on sequence similarity, the antibody may cross-react with CDK2 and CDK3. Phospho-cdc2 (Thr14) Antibody能够检测内源性苏氨酸(14位)磷酸化的cdc2 (CDK1)蛋白。由于序列相似性,该抗体可能与CDK2和CDK3发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr14 of human cdc2. Antibodies are purified by protein A and peptide affinity chromatography. 该单克隆抗体是由合成的人源的针对cdc2蛋白苏氨酸(14位)磷酸化肽段免疫动物,利用A蛋白和多肽亲和层析方法纯化生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from hydroxyurea-treated HT29 cells, aphidicolin-treated HeLa cells, and asynchronous and mitotic CHO cells using Phospho-cdc2 (Thr14) Antibody. Western blot方法检测细胞提取物:羟基脲处理的HT29细胞,阿非迪霉素处理的HeLa细胞及非同步的和有丝分裂的CHO细胞,使用的抗体为Phospho-cdc2 (Thr14) Antibody。

Western Blotting

Western Blotting

Western blot analysis of extracts from asynchronous and mitotic HeLa cells using Phospho-cdc2 (Thr14) Antibody. Western blot方法检测非同步和有丝分裂HeLa细胞提取物,使用的抗体为Phospho-cdc2 (Thr14) Antibody。

Background

The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5). cdc2蛋白激酶的激活参与调节真核细胞进入有丝分裂,cdc2的激活受多个步骤的调控,包括周期蛋白的结合、cdc2苏氨酸161位的磷酸化(1)。然而,在细胞进入有丝分裂的过程中,激活cdc2的决定性步骤是苏氨酸14位和15位的去磷酸化(2)。Wee1 和 Myt1蛋白激酶通过磷酸化cdc2苏氨酸14位和15位,抑制cdc2活性(3,4)。而cdc25磷酸酯酶可能起到使cdc2苏氨酸14位和15位去磷酸化从而激活cdc2的作用(1,5)。

  1. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
  2. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
  3. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
  4. Wells, N.J. et al. (1999) J Cell Sci 112 ( Pt 19), 3361-71.
  5. Hunter, T. (1995) Cell 80, 225-36.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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