Cell Signaling Technology

Product Pathways - Adhesion

Phospho-Paxillin (Tyr118) Antibody #2541

No. Size Price
2541S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2541 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,Pig, Endogenous 68 Rabbit
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Zebrafish,

Specificity / Sensitivity

Phospho-Paxillin (Tyr118) Antibody detects endogenous levels of paxillin only when phosphorylated at tyrosine 118. The antibody does not cross-react with other tyrosine phosphorylated proteins.

Phospho-Paxillin (Tyr118)抗体仅能识别在118位酪氨酸磷酸化后的内源性paxillin蛋白。抗体与其他酪氨酸磷酸化的蛋白没有交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr118 of human paxillin. Antibodies are purified by protein A and peptide affinity chromatography.

合成对应人Paxillin蛋白118位酪氨酸及其邻近氨基酸残基序列的多肽免疫动物获得多克隆抗体。抗体经过蛋白A和肽亲和层析纯化。

IF-IC

IF-IC

Confocal immunofluorescent analysis of Hela cells -/+ lamda phosphatse labeled with Phospho-Paxillin (Tyr118) Antibody (green). Note the robust signal in the untreated cells (left) versus the lambda phosphatse treated cells (right).Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). Red = phalloidin555.使用Phospho-Paxillin (Tyr118) 抗体(绿色)对-/+ lamda磷酸酶标记的HeLa细胞进行激光共聚焦免疫荧光分析。注意相对lambda磷酸酶处理(右)细胞未处理细胞(左)中强烈的信号。蓝色假色=DRAQ5™(DNA荧光燃料)。红色=鬼笔环肽555.

Western Blotting

Western Blotting

Western blot analysis of untreated and lambda-phosphatase treated COS cells, using Phospho-Paxillin (Tyr118) Antibody (left) and Paxillin Antibody #2542 (right).使用Phospho-Paxillin (Tyr118) 抗体(左)和Paxillin抗体#2542(右)对未处理或经过lambda磷酸酶处理的COS细胞提取物进行western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Phospho-Paxillin (Tyr118) Antibody.使用Phospho-Paxillin (Tyr118) 抗体对多种细胞提取物进行western blot分析。

Background

Paxillin is a multi-domain protein that localizes primarily to focal adhesion sites to the extracellular matrix (1). Paxillin is one of the key components of integrin signaling and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at Tyr118 (3,4). Phospho-Tyr118 may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5).

Paxillin是一个多结构域蛋白,位于黏着斑面对细胞外基质的部位(1)。Paxillin是整合素信号一个重要组成部分,Paxillin酪氨酸磷酸化对于整合素介导的细胞骨架重构是必需的(2)。Paxillin由另一个黏着斑组分磷酸化118位酪氨酸,粘着激酶(FAk)(3,4)。118位酪氨酸磷酸化为其他信号分子聚集到黏着斑提供了接头。已有结果显示Crk的SH2结构域能够与Paxillin磷酸化的118位酪氨酸结合(5)。

  1. Turner, C.E. (2000) J Cell Sci 113 Pt 23, 4139-40.
  2. Burridge, K. et al. (1992) J Cell Biol 119, 893-903.
  3. Bellis, S.L. et al. (1995) J Biol Chem 270, 17437-41.
  4. Bellis, S.L. et al. (1997) Biochem J 325 ( Pt 2), 375-81.
  5. Schaller, M.D. and Parsons, J.T. (1995) Mol Cell Biol 15, 2635-45.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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