Cell Signaling Technology

Product Pathways - Protein Stability

Calpain 2 Large Subunit (M-type) Antibody #2539

Calcium-activated-neutral-proteinase-2   Calpain-2-catalytic-subunit   Calpain-2-large-subunit   Calpain-large-polypeptide-L2   Calpain-M-type   CAN2   CANP-2   CANPL2   CAPN2   M-calpain   Millimolar-calpain  

No. Size Price
2539S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
2539 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 80 Rabbit
IP 1:50
F 1:25
IF-F 1:25

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

Calpain 2 Large Subunit (M-type) Antibody detects endogenous levels of total calpain 2 (large subunit) protein. The antibody detects full-length calpain 2 as well as calpain 2 autoproteolytically cleaved at serine 20. The antibody does not detect recombinant calpain 1.

Calpain 2 Large Subunit (M-type)抗体可以识别内源性总的calpain2(大亚基)蛋白。抗体可以是被全长的钙蛋白酶2和在20位丝氨酸处进行了自发蛋白水解的calpain2蛋白。抗体不能识别重组钙蛋白酶1。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human sequence of calpain 2 (large subunit). Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 and PC3 cells, using Calpain 2 Large Subunit (M-type) Antibody.使用Calpain 2 Large Subunit (M-type) 抗体对C2C12和PC3细胞提取物进行western blot分析。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of C2C12 cells, using Calpain 2 Large Subunit (M-type) Antibody (blue) compared to a nonspecific negative control antibody (red).使用Calpain 2 Large Subunit (M-type) 抗体(蓝色)对比非特异性阴性对照抗体(红色)对C2C12细胞进行流式细胞分析。



Confocal immunofluorescent analysis of mouse retina using Calpain 2 Large Subunit (M-type) Antibody (red) and Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Calpain 2 Large Subunit (M-type) 抗体(红色)和Phospho-Tyrosine 小鼠单抗(P-Tyr-100) #9411(绿色)对小鼠视网膜进行激光共聚焦免疫荧光分析。蓝色假色=DRAQ5® #4084 (DNA荧光染料)。


Calpain is a calcium-dependent thiol proteinase that is functionally active as a heterodimer composed of a small regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation. The regulation of calpain in vivo is the subject of many current studies, which suggest that proteolytic activity is regulated post-transcriptionally by mechanisms such as calcium requirements, subcellular localization of the heterodimer, phosphorylation via the EGFR-Erk signaling cascade, endogenous inhibitors (calpastatin) and autoproteolytic cleavage (1). Calpastatin negatively regulates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28 (2). Calpain influences cell migration by modifying rather than degrading its substrates responsible for cell adhesion and cytoskeletal arrangement. Control of calpain activity has caught the attention of drug development since limiting its activity could mute invasiveness of tumors or chronic inflammation (1).


  1. Perrin, B.J. and Huttenlocher, A. (2002) Int. J. Biochem. Cell Biol. 34, 722-725.
  2. Melloni, E. et al. (1996) Biochem. Biophys. Res. Commun. 229, 193-197.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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