Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Acetyl-Stat3 (Lys685) Antibody #2523

No. Size Price
2523L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
2523S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2523 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 79, 86 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Acetyl-Stat3 (Lys685) Antibody detects transfected Stat3 when acetylated at lysine 685.

Acetyl-Stat3 (Lys685)抗体只能检测转染水平的在lys685位点乙酰基化的Stat3蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding lysine 685 of Stat3. Antibodies were purified by protein A and peptide affinity chromatography.

多克隆抗体是通过合成对应的Stat3 Lys685位点周围乙酰基化的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells transfected with Stat3, CBP or both, using Acetyl-Stat3 (Lys685) Antibody (top) or total Stat3 Antibody #9139.Western免疫印迹。用 Acetyl-Stat3 (Lys685) Antibody (上图) 或 total Stat3 Antibody #9139研究转染了Stat3, CBP 或者两个共转的HeLa细胞的细胞提取液。

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

Stat3 转录因子是细胞因子和生长因子受体信号通路中重要的信号分子(1),并且对于小鼠胎儿发育是必须的(2)。Stat3 在很多的人类肿瘤中是组成性激活的(3,4)并有潜在的支配原发性肿瘤的可能(5) ,而且也具有抗凋亡活性(3)。Stat3通过Tyr705位点的磷酸化而激活,从而导致它的二聚化,入核后与DNA结合(6,7)。转录活性似乎受到 MAPK 或 mTOR 信号通路介导的Stat3 Ser727位点的磷酸化的调控(8,9)。Stat3不同剪切形式的表达反应了其生物学功能,Stat3α (86 kDa) 和 Stat3β (79 kDa) 形式的表达比例依赖于细胞类型、配体方向或者细胞发育的成熟阶段(10)。值得注意的是,Stat3β蛋白在C-末端转录活性区域缺少丝氨酸磷酸化位点(8)。

In addition to phosphorylation, Stat3 can be modified by acetylation. Stat3 is acetylated at Lys685 by p300/CREB-binding protein (CBP) which can stimulate DNA binding and transactivation activity (11,12).

除了磷酸化, Stat3也能被乙酰基化修饰。Stat3通过p300/CREB结合蛋白(CBP)在Lys685位点乙酰基化,CBP能够刺激DNA结合和转录活性(11,12)。

  1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
  2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
  3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
  6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
  7. Ihle, J.N. (1995) Nature 377, 591-4.
  8. Wen, Z. et al. (1995) Cell 82, 241-50.
  9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
  10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.
  11. Yuan, Z.L. et al. (2005) Science 307, 269-73.
  12. Wang, R. et al. (2005) J. Biol. Chem. 280, 11528-34.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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