Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-FoxO1 (Ser319) Antibody #2486

FKHR   Forkhead box protein O1A   Forkhead in rhabdomyosarcoma   Fox01   foxo 1a   foxo1 a   FoxO1A   sc-101682  

No. Size Price
2486S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2486 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 120 (GFP-FoxO1) Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-FoxO1 (Ser319) Antibody detects exogenous levels of FoxO1 only when phosphorylated at serine 319. The antibody does not cross-reacts with FoxO1 phosphorylated at other sites nor with other phosphorylated family members.

Phospho-FoxO1 (Ser319)抗体能够检测在Ser319位点磷酸化的外源性的FoxO1 蛋白。此抗体不和其他位点磷酸化的FoxO1蛋白发生交叉反应,也不和其它磷酸化的家族蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser319 of human FoxO1. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是由合成的人源的针对FoxO1 蛋白Ser319位的磷酸化肽段免疫动物而生产的。抗体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated or serum-treated COS-7 cells exogenously expressing GFP-FoxO1, using Phospho-FoxO1 (Ser319) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after Western transfer.

Western blot方法检测分析未处理和血清处理的外源性表达GFP-FoxO1蛋白的COS-7细胞株提取物,使用的抗体是Phospho-FoxO1 (Ser319)。Western转膜后,分别用小牛肠碱性磷酸酶 (CIP) 处理和不处理的膜确认抗体识别磷酸化蛋白的特异性。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-treated COS-7 cells untransfected, exogenously expressing FoxO4 or GFP-FoxO1, using Phospho-FoxO1 (Ser319) (left) or Phospho-FoxO1 (Ser319)/FoxO4 (Ser262) Antibody #2487 (right).

Western blot方法检测分析血清处理的外源性表达FoxO4 和GFP-FoxO1蛋白的未转染处理的COS-7细胞株提取物,使用的抗体是Phospho-FoxO1 (Ser319)(左图)和Phospho-FoxO1 (Ser319)/FoxO4 (Ser262) Antibody #2487(右图)。

Background

The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).

Forkhead转录因子家族参与横纹肌肉瘤和急性白血病的发生(1-3)。在这个家族中,三个成员(FoxO1,FoxO4和FoxO3a)与线虫的同源基因DAF-16具有序列相似度,而DAF-16介导包括IGFR1,PI3K和Akt在内的信号通路(4-6)。活化的forkhead成员作为肿瘤抑制因子,促进细胞周期阻滞和凋亡。FOXO家族任何成员的表达上调都会激活细胞周期抑制剂p27 Kip1。Forkhead转录因子也参与TGF-β介导的p21 CIP1上调,而这个上调过程可以被PI3K负调控(7)。forkhead转录因子被Akt在Thr24,Ser256以及Ser319磷酸化后而失活,导致出核转运与转录因子活性的抑制(8),最终导致增殖上调。Forkhead转录因子活性也可以被去乙酰化酶sirtuin(SirT1)抑制(9)。

  1. Anderson, M.J. et al. (1998) Genomics 47, 187-99.
  2. Galili, N. et al. (1993) Nat Genet 5, 230-5.
  3. Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
  4. Nakae, J. et al. (1999) J Biol Chem 274, 15982-5.
  5. Rena, G. et al. (1999) J Biol Chem 274, 17179-83.
  6. Guo, S. et al. (1999) J Biol Chem 274, 17184-92.
  7. Seoane, J. et al. (2004) Cell 117, 211-23.
  8. Arden, K.C. (2004) Mol Cell 14, 416-8.
  9. Yang, Y. et al. (2005) EMBO J 24, 1021-32.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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