Cell Signaling Technology

Product Pathways - Neuroscience

Cystatin C (D6U3E) Rabbit mAb #24840

No. Size Price
24840S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
24840 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 13 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Cystatin C (D6U3E) Rabbit mAb recognizes endogenous levels of total cystatin C protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human cystatin C protein.

IP

IP

Immunoprecipitation of cystatin C from Huh7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Cystatin C (D6U3E) Rabbit mAb (lane 3). Western blot analysis was performed using Cystatin C (D6U3E) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Cystatin C (D6U3E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human cystatin C protein (hCysC-Myc; +), using Cystatin (D6U3E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Cystatin C (cystatin-3, CST3) belongs to the cystatin type 2 superfamily of cysteine peptidase inhibitors (1). The 146 amino acid cystatin C precursor protein contains a 26 residue, amino-terminal secretory signal sequence. The mature 120 amino acid cystatin C protein contains two disulfide bridges (2,3). Cystatin C is secreted in body fluids and is a marker of kidney (4) and cardiovascular (5) dysfunction. Research studies report of changes in cystatin C levels in the cerebral spinal fluid as well as in specific neuronal cell populations in a number of neurodegenerative diseases (6-8). Interestingly, experimental evidence suggests that cystatin C has protective effects against neurodegeneration, such as inhibition of amyloid-β oligomerization and fibril formation (9,10), induction of autophagy (11), induction of neurogenesis (12), and inhibition of cysteine proteases whose activity has been associated with several neurodegenerative diseases (13).

  1. Barrett, A.J. (1986) Biomed Biochim Acta 45, 1363-74.
  2. Turk, V. and Bode, W. (1991) FEBS Lett 285, 213-9.
  3. Turk, B. et al. (1997) Biol Chem 378, 141-50.
  4. Filler, G. et al. (2005) Clin Biochem 38, 1-8.
  5. Wang, G.N. et al. (2014) Clin Biochem 47, 176-81.
  6. Pasinetti, G.M. et al. (2006) Neurology 66, 1218-22.
  7. Deng, A. et al. (2001) Am J Pathol 159, 1061-8.
  8. Ishimaru, H. et al. (1996) Brain Res 709, 155-62.
  9. Sastre, M. et al. (2004) Neurobiol Aging 25, 1033-43.
  10. Selenica, M.L. et al. (2007) Scand J Clin Lab Invest 67, 179-90.
  11. Tizon, B. et al. (2010) PLoS One 5, e9819.
  12. Pirttilä, T.J. et al. (2005) Neurobiol Dis 20, 241-53.
  13. Kaur, G. et al. (2010) Am J Pathol 177, 2256-67.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications