Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-PLCbeta3 (Ser537) Antibody #2481

phospholipase-C   PLC   PLC-beta   PLC-beta-3   PLCbeta   PLCbeta3  

No. Size Price
2481S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2481 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 150 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-PLCBeta3 (Ser537) Antibody detects endogenous levels of PLCbeta3 only when phosphorylated at serine 537. The antibody does not cross-react with phosphorylated PLCbeta1, PLCbeta2, PLCbeta4 or other PLCs.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding residues surrounding Ser537 of human PLCbeta3. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells treated with anisomycin (20 micrograms/ml) for 0, 15, 30 and 45 minutes, using Phospho-PLCbeta3 (Ser537) Antibody.

对20mg/ml anisomycin处理0,15,30和45分钟的HeLa细胞抽提液,使用Phospho-PLCbeta3 (Ser537)抗体进行Western blot分析。


Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3). 
 Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLCβ3 activity (4,5). Ser537 of PLCβ3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLCβ3. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (6). 
 PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1245 (7). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (8).


  1. Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509.
  2. Smrcka, A.V. et al. (1991) Science 251, 804-7.
  3. Taylor, S.J. et al. (1991) Nature 350, 516-8.
  4. Yue, C. et al. (1998) J Biol Chem 273, 18023-7.
  5. Yue, C. et al. (2000) J Biol Chem 275, 30220-5.
  6. Margolis, B. et al. (1989) Cell 57, 1101-7.
  7. Kim, H.K. et al. (1991) Cell 65, 435-41.
  8. Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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