Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-VEGF Receptor 2 (Tyr996) Antibody #2474

flk-1   kdr   kinase insert domain receptor   vascular endothelial growth factor   vegf receptor   vegf receptor 2   vegf receptor-2   vegf receptor2   vegfr   vegfr 2   vegfr-2   vegfr2  

No. Size Price
2474S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2474T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
2474 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Transfected Only 230 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-VEGF Receptor 2 (Tyr996) Antibody detects endogenous VEGFR 2 only when phosphorylated at tyrosine 996. The antibody cross-reacts with tyrosine-phosphorylated PDGF receptor.

磷酸化VEGF Receptor 2 (Tyr996) Antibody 仅在Tyr 996磷酸化时才能检测内源VEGFR2 蛋白的水平。本抗体与酪氨酸磷酸化PDGF receptor交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr996 of human VEGF receptor 2. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体用合成的磷酸化多肽免疫动物得到,该多肽根据人VEGF receptor 2 的Tyr996附近的氨基酸合成。抗体经过protein A和亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGF receptor 2 intracellular domains (Rahimi, N. et al. (2000) J. Biol. Chem. 275, 16986-16992), using Phospho-VEGF Receptor 2 (Tyr996) Antibody (upper) or VEGF receptor 2 antibody (lower). 用Phospho-VEGF Receptor 2 (Tyr996) Antibody (上) 或VEGF receptor 2 antibody (下)对含有人CSF-1胞外结合区/小鼠VEGF receptor 2胞内区的嵌合受体的CKR/PAE细胞的提取物进行免疫印迹检测(Rahimi, N. et al. [2000] J. Biol. Chem. 275, 16986-16992)。

Background

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).

血管内皮生长因子受体2 (VEGFR2, KDR, Flk-1)是内皮细胞上VEGF诱导信号时的一个主要受体。和配体结合之后,VEGFR2发生自磷酸化并被活化(1)。VEGFR2 主要的自磷酸化受体位于激酶插入区 (Tyr951/996)和酪氨酸激酶催化区(Tyr1054/1059) (2)。受体的激活会导致对包括Shc, Grb2, PI3K激酶, Nck 和蛋白酪氨酸磷酸化酶SHP-1 和 SHP-2接头蛋白的快速招募(3)。Tyr1212的磷酸化为Grb2提供了结合位点而且磷酸化的Tyr1175能结合PI3K激酶的p85 亚基以及PLCγ和 Shb (1,4,5)。VEGFR2信号传导对于实现VEGF刺激引起的增殖、趋化和发芽,以及体外培养的内皮细胞的增殖和体内血管的发生是必需的(6-8)。

  1. Meyer, M. et al. (1999) EMBO J 18, 363-74.
  2. Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.
  3. Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.
  4. Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
  5. Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
  6. Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
  7. Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
  8. Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.

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