Product Pathways - Cytoskeletal Signaling
Phospho-Rac1/cdc42 (Ser71) Antibody #2461
|2461S||100 µl ( 10 western blots )||￥4,050.00 现货查询||购买询价|
|2461T||20 µl ( 2 western blots )||￥1,500.00 现货查询||购买询价|
|2461||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting,
Species predicted to react based on 100% sequence homology: Mouse, Rat,
Specificity / Sensitivity
Phospho-Rac1/cdc42 (Ser71) Antibody detects endogenous levels of Rac1/cdc42 only when phosphorylated at serine 71. The antibody may also recognize phospho-RhoA (Ser73).
Phospho-Rac1/cdc42 (Ser71) Antibody检测仅在serine 71位点磷酸化的内源性Rac1/cdc42蛋白。该抗体也可能识别phospho-RhoA (Ser73)蛋白。
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser71 of human Rac1/cdc42. Antibodies are purified by protein A and peptide affinity chromatography.
Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development (1). GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein's intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state (2,3).
Rac和Cdc42蛋白是Rho-GTPase家族成员。在哺乳动物类，Rac蛋白存在有三个亚型Rac1、Rac2和Rac3，它们在序列上高度类似。Rac1和Cdc42蛋白是在这个家族中最广泛研究的，它们无处不在的表达。Rac2蛋白在造血起源的细胞中被表达，在大脑中高表达的Rac3蛋白也被发现在许多其它组织。Rac和Cdc42蛋白在细胞骨架的重组、膜运输、转录调控、细胞生长和发育中起到关键的信号角色(1)。GTP的结合刺激Rac/Cdc42蛋白的活性，然而通过蛋白质内部GTPase活性促使GTP水解成GDP，致使它的失活。GTPase激活蛋白(GAPs)辅助GTP的水解，而鸟嘌呤核苷交换因子（guanine nucleotide exchange factors ，GEFs）有助于GDP的转换成GTP 。通过鸟苷酸解离抑制因子(RhoGDI)的结合使得达到另一个调节的水平，这保留了Rho家族GTPases包括Rac和Cdc42，在它们失活的GDP结合水平(2,3)。
A putative Akt phosphorylation site at Ser71 of Rac1/cdc42 has been identified and confirmed by in vitro kinase assay (4). Phosphorylation at this site may inhibit GTP binding of Rac1, attenuating the signal transduction pathway downstream of Rac1 (4).
- Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12.
- Bernards, A. and Settleman, J. (2004) Trends Cell Biol 14, 377-85.
- Rossman, K.L. et al. (2005) Nat Rev Mol Cell Biol 6, 167-80.
- Kwon, T. et al. (2000) J. Biol. Chem. 275, 423-428.
- John, G. R. et al. (2004) . J. Neurosci. 24 (11), 2837-2845.Applications:Western Blotting,
- Faraone, D. et al. (2006) Blood 107, 1896-902.Applications:Western Blotting,
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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