Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-eIF4G (Ser1108) Antibody #2441

eIF   elF4G   initiation  

No. Size Price
2441S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2441T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
2441 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster,Monkey,Bovine, Endogenous 220 Rabbit
IP 1:100
IF-IC 1:600

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-eIF4G (Ser1108) Antibody detects eIF4GI only when phosphorylated at Ser1108. It does not cross-react with nonphosphorylated eIF4GI or p97.

Phospho-eIF4G (Ser1108) Antibody检测仅在Ser1108位点磷酸化的内源性eIF4GI蛋白。该抗体不与非磷酸化的eIF4GI或p97发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1108 of human eIF4GI. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from PC12 cells, untreated (lane 1), NGF-treated (10 ng/ml) (lane 2), anisomycin-treated (25 µM) (lane 3), U0126-treated #9903 (10 µM) (lane 4), Rapamycin-treated #9904 (100 nM ) (lane 5) or LY294002-treated #9901 (25 µM) (lane 6), using Phospho-eIF4G (Ser1108) Antibody.

使用Phospho-eIF4G (Ser1108) Antibody,免疫印迹(Western Blot)分析在PC12细胞系中Phospho-eIF4G (Ser1108)蛋白水平,分别分为对照组(第1道),NGF处理组(10 ng/ml) (第2道),anisomycin处理组(25 µM) (第3道),U0126处理组 #9903 (10 µM) (第4道),Rapamycin处理组#9904 (100 nM ) (第5道)和LY294002处理组 #9901 (25 µM) (第6道)。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells expressing GST-eIF4GI Ser1192Ala or GST-eIF4GI Ser1108Ala mutant protein, using Phospho-eIF4G (Ser1108) Antibody. (Provided by Brian Raught, McGill University, Montreal, QuŽbec.)

使用Phospho-eIF4G (Ser1108) Antibody,免疫印迹(Western Blot)分析在293细胞系中GST-eIF4GI Ser1192Ala和GST-eIF4GI Ser1108Ala突变体蛋白的水平。(Provided by Brian Raught, McGill University, Montreal, QuŽbec.)



Confocal immunofluorescent analysis of HeLa cells either rapamycin-treated (left) or serum-treated (right), using Phospho-eIF4G (Ser1108) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

使用Phospho-eIF4G (Ser1108) Antibody(绿色),共聚焦免疫荧光观察Phospho-eIF4G (Ser1108)蛋白在HeLa细胞中定位,细胞分为rapamycin处理组(左图)和血清处理组(右图)。DY-554 phalloidin (红色)标记微丝蛋白。蓝色伪彩= DRAQ5™ (DNA荧光染料)


Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.

Eukaryotic initiation factor 4E (eIF4E)结合mRNA帽子结构从而介导翻译的起始(1,2)。eIF4E和eIF4G相互作用,而eIF4G是一个支架蛋白其能促进eIF4E和eIF4A装配到eIF4F复合物上(2)。eIF4B被认为在翻译起始阶段有助于eIF4F复合物的形成。在有丝分裂和Erk、p38 MAPK介导的压力刺激下,体内Mnk1蛋白使eIF4E蛋白在Ser209位点磷酸化(3,4)。在小鼠Mnk1上的两个Erk和p38 MAPK磷酸化位点(Thr197 and Thr202)对Mnk1激酶活性至关重要(3)。eIF4G蛋白羧基末端含有血清刺激的磷酸化位点,包括Ser1108、Ser1148和Ser1192 (5)。通过PI3激酶抑制剂LY294002和FRAP/mTOR通路抑制剂rapamycin可阻断这些位点的磷酸化。

  1. Sonenberg, N. et al. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847.
  2. Gingras, A.C. et al. (1999) Annu. Rev. Biochem. 68, 913-963.
  3. Waskiewicz, A. et al. (1999) Mol. Cell. Biol. 19, 1871-1880.
  4. Pyronnet, S. et al. (1999) EMBO J. 18, 270-279.
  5. Raught, B. et al. (2000) EMBO J. 19, 434-444.

Application References

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