Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Shc (Tyr239/240) Antibody #2434

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No. Size Price
2434S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2434T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
2434 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 50, 55, 70 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Shc (Tyr239/240) Antibody detects Shc only when phosphorylated at tyrosine 239/240. The antibody may cross-react with activated EGF receptor protein.

Phospho-Shc (Tyr239/240) Antibody能检测酪氨酸(239/240位点)磷酸化的Shc 蛋白水平。该抗体可能与活化的EGF受体蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr239/240 of human Shc. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体通过使用与人源Shc蛋白酪氨酸(239/240位点)周围残基相一致的合成磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HepG2 cells, untreated or EGF-treated (100 ng/ml) 18 hours of serum-starvation, and Jurkat cells, untreated or treated with anti-CD3 antibody (1 µg/ml for 10 minutes), using Phospho-Shc (Tyr239/240) Antibody (upper) or Shc antibody #2432 (lower).Western blot方法检测细胞提取物:未经处理的和EGF (100 ng/ml)处理的18h无血清培养HepG2细胞,未处理和抗CD3抗体(1 µg/ml 10 min)处理的Jurkat细胞 。使用的抗体是Phospho-Shc (Tyr239/240) Antibody (上图)和Shc antibody #2432(下图).

Background

Shc possesses SH2 and PTB domains and serves as a scaffold protein in signaling for a variety of receptor tyrosine kinases. Shc exists in p46, p52 and p66 isoforms, which are produced by using alternative translation initiation sites or a differentially spliced message (1-3). In response to extracellular signals, the SH2 and PTB domains of Shc interact with the activated receptors, leading to phosphorylation of Shc on three different tyrosine residues: Tyr239, Tyr240 and Tyr317 (4-6). GRB2/Sos binds to Shc phosphorylated at these sites, activating the Ras/Raf/MAPK pathway (4). Both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development (7).

Shc包含SH2和PTB结构域,并且是各种受体酪氨酸激酶信号转导过程中的骨架蛋白。Shc以p46, p52,p66异构体的形式存在,这是使用选择性转位抑制位点、不同剪接信息的结果(1-3)。应答细胞内信号,Shc蛋白的SH2和PTB结构域与活化的受体相互作用,导致Shc蛋白三个不同酪氨酸(239/240/317位点)的磷酸化(4-6)。GRB2/Sos蛋白结合酪氨酸(239/240/317位点)磷酸化的Shc蛋白,激活Ras/Raf/MAPK通路(4)。Shc蛋白的表达以及它的酪氨酸位点磷酸化在胸腺T细胞的发育中起着重要且不可或缺的作用(7)。

  1. Pelicci, G. et al. (1992) Cell 70, 93-104.
  2. Migliaccio, E. et al. (1997) EMBO J. 16, 706-716.
  3. Okada, S. et al. (1997) J. Biol. Chem. 272, 28042-28049.
  4. Salcini, A. E. et al. (1994) Oncogene 9, 2827-2836.
  5. Gotoh, N. et al. (1996) EMBO J. 15, 6197-6204.
  6. Van der Geer, P. et al. (1996) Curr. Biol. 6, 1435-1444.
  7. Zhang, L. et al. (2002) Nat. Immunol. 3, 749-755.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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