Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Shc (Tyr317) Antibody #2431


No. Size Price
2431S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2431 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 50, 55, 70 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Shc (Tyr317) Antibody detects Shc only when phosphorylated at Tyr317.

Phospho-Shc (Tyr317) Antibody兔多抗能检测酪氨酸(371位点)磷酸化的Shc 蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr317 of human Shc. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from A431 and HepG2 cells, untreated or EGF-treated (100 ng/ml) after 24 hours of serum-starvation, using Phospho-Shc (Tyr317) Antibody (upper) or Shc antibody (lower).Western blot方法检测细胞提取物:未经处理的和EGF (100 ng/ml)处理的24h无血清培养A431、 HepG2细胞。使用的抗体是Phospho-Shc (Tyr317) Antibody(上图)和Shc antibody(下图).


Shc possesses SH2 and PTB domains and serves as a scaffold protein in signaling for a variety of receptor tyrosine kinases. Shc exists in p46, p52 and p66 isoforms, which are produced by using alternative translation initiation sites or a differentially spliced message (1-3). In response to extracellular signals, the SH2 and PTB domains of Shc interact with the activated receptors, leading to phosphorylation of Shc on three different tyrosine residues: Tyr239, Tyr240 and Tyr317 (4-6). GRB2/Sos binds to Shc phosphorylated at these sites, activating the Ras/Raf/MAPK pathway (4). Both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development (7).

Shc包含SH2和PTB结构域,并且是各种受体酪氨酸激酶信号转导过程中的骨架蛋白。Shc以p46, p52,p66异构体的形式存在,这是使用选择性转位抑制位点、不同剪接信息的结果(1-3)。应答细胞内信号,Shc蛋白的SH2和PTB结构域与活化的受体相互作用,导致Shc蛋白三个不同酪氨酸(239/240/317位点)的磷酸化(4-6)。GRB2/Sos蛋白结合酪氨酸(239/240/317位点)磷酸化的Shc蛋白,激活Ras/Raf/MAPK通路(4)。Shc蛋白的表达以及它的酪氨酸位点磷酸化在胸腺T细胞的发育中起着重要且不可或缺的作用(7)。

  1. Pelicci, G. et al. (1992) Cell 70, 93-104.
  2. Migliaccio, E. et al. (1997) EMBO J. 16, 706-716.
  3. Okada, S. et al. (1997) J. Biol. Chem. 272, 28042-28049.
  4. Salcini, A. E. et al. (1994) Oncogene 9, 2827-2836.
  5. Gotoh, N. et al. (1996) EMBO J. 15, 6197-6204.
  6. Van der Geer, P. et al. (1996) Curr. Biol. 6, 1435-1444.
  7. Zhang, L. et al. (2002) Nat. Immunol. 3, 749-755.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :