Product Pathways - Tyrosine Kinase / Adaptors
Phospho-PDGF Receptor α (Tyr762) (D9B1N) Rabbit mAb #24188
|24188S||100 µl ( 10 western blots )||￥4,050.00 现货查询||购买询价|
|24188||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Species predicted to react based on 100% sequence homology: Mouse, Rat,
Specificity / Sensitivity
Phospho-PDGf Receptor α (Tyr762) (D9B1N) Rabbit mAb recognizes endogenous levels of PDGF Receptor α protein only when phosphorylated at Tyr762. The antibody might cross react slightly with other overexpressed phosphorylated receptor tyrosine kinase such as EGFR.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr762 of human PDGF Receptor α protein.
Immunoprecipitation of Phospho-PDGF Receptor α (Tyr762) protein from Human Platelet-Derived Growth Factor AA (hPDGF-AA) #8913 treated (100 ng/ml, 5 min) NCI-H1703 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-PDGF Receptor α (Tyr762) (D9B1N) Rabbit mAb. Western blot analysis was performed using Phospho-PDGF Receptor α (Tyr762) (D9B1N) Rabbit mAb (multiple partially degraded phospho-PDGF Receptor α bands shown at lower molecular size).
Western blot analysis of extracts from NCI-H1703 cells serum-starved overnight and then either untreated (-) or treated with Human Platelet-Derived Growth Factor AA (hPDGF-AA) #8913 (100 ng/ml, 10 min;+), using Phospho-PDGF Receptor α (Tyr762) Rabbit mAb (upper) or PDGF Receptor α (D13C6) XP® Rabbit mAb #5241 (lower).
Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRα homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRβ homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor α/β binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCγ, and NCK. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5).
Phosphorylation of PDGFRα at Tyr762 was identified at Cell Signaling Technology using PTMScan®, our LC-MS/MS platform for phosphorylation site discovery (6). Tyr762 is located in the activation loop of the PDGFRα kinase domain. Phosphorylation of PDGFRα at this site was also reported by several other labs to be a docking site for CrkII and CrkL upon induction by growth factor treatment (7,8).
- Deuel, T.F. et al. (1988) Biofactors 1, 213-7.
- Bergsten, E. et al. (2001) Nat Cell Biol 3, 512-6.
- Betsholtz, C. et al. (2001) Bioessays 23, 494-507.
- Coughlin, S.R. et al. (1988) Prog Clin Biol Res 266, 39-45.
- Ostman, A. and Heldin, C.H. (2001) Adv Cancer Res 80, 1-38.
- Rikova, K. et al. (2007) Cell 131, 1190-203.
- Matsumoto, T. et al. (2000) Biochem Biophys Res Commun 270, 28-33.
- Yokote, K. et al. (1998) Oncogene 16, 1229-39.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
PTMScan is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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