Cell Signaling Technology

Product Pathways - Metabolism

OGT (D1D8Q) Rabbit mAb #24083

FLJ23071   HRNT1   MGC22921   O-GLCNAC   O-GlcNAc transferase p110 subunit   O-GlcNAc transferase subunit p110   O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)   O-linked N-acetylglucosamine transferase 110 kDa subunit   OGT   OGT1   UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit   uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase  

No. Size Price
24083S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
24083 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 110 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

OGT (D1D8Q) Rabbit mAb recognizes endogenous levels of total OGT protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro546 of human OGT protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using OGT (D1D8Q) Rabbit mAb.

Background

O-GlcNAcylation is a post-translational modification where β-D-N-acetylglucosamine (GlcNAc) is covalently linked to cytoplasmic and nuclear proteins at serine or threonine residues (1,2). This modification is important in many cellular processes including metabolism, cell growth and morphogenesis, apoptosis, and transcription (2,3), and research studies have implicated this modification in cancer (1). The reversible protein modification by O-GlcNAc, which has been suggested to be a nutrient and stress sensor, is catalyzed by two highly conserved enzymes, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) (4).

  1. Comer, F.I. et al. (2001) Anal Biochem 293, 169-77.
  2. Slawson, C. and Hart, G.W. (2011) Nat Rev Cancer 11, 678-84.
  3. Capotosti, F. et al. (2011) Cell 144, 376-88.
  4. Hart, G.W. et al. (2007) Nature 446, 1017-22.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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