Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-HSP27 (Ser82) Antibody II #2406

No. Size Price
2406S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2406T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
2406 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 27 Rabbit
IHC-P 1:100
F 1:50
IF-IC 1:400
IHC-F 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-F=Immunohistochemistry (Frozen),

Specificity / Sensitivity

Phospho-HSP27 (Ser82) Antibody II detects endogenous HSP27 only when phosphorylated at Ser82. The antibody does not recognize other heat shock proteins.

Phospho-HSP27 (Ser82) Antibody II识别内源性的Ser82磷酸化的HSP27蛋白。此抗体不与其它HSF蛋白相互作用。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser82 of human HSP27. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体用与人类HSP27中Ser82位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, untreated (blue) or UV-treated (green), using Phospho-HSP27 (Ser82) Antibody II.

对未处理(蓝)或紫外线处理(绿)的Hela细胞使用Phospho-HSP27 (Ser82) Antibody II进行流式细胞术分析。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using Phospho-HSP27 (Ser82) Antibody II (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

对HeLa细胞,未处理(左)或anisomycin处理(右),使用Phospho-HSP27 (Ser82) Antibody II(绿色)进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor®555 phalloidin(红色)标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-HSP27 (Ser82) Antibody II.

对石蜡包埋的人肺癌使用Phospho-HSP27 (Ser82) Antibody II进行免疫组化分析。

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen H1650 xenograft using Phospho-HSP27 (Ser82) Antibody II.

对冰冻的H1650异种移植物使用Phospho-HSP27 (Ser82) Antibody II进行免疫组化分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and PC12 cells, treated with λ-phosphatase or UV as indicated, using Phospho-HSP27 (Ser82) Antibody II.

对HeLa和PC12细胞,按要求进行λ磷酸酶或紫外线处理,使用Phospho-HSP27 (Ser82) Antibody II进行Western blot分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human melanoma using Phospho-HSP27 (Ser82) Antibody II.

对石蜡包埋的人黑色素瘤使用Phospho-HSP27 (Ser82) Antibody II进行免疫组化分析。

Background

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78 and Ser82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).

热休克蛋白(HSP)27是小HSP之一,在多种细胞和组织中都有不同水平的组成性表达。像其它小HSP一样,HSP27的调节也发生在转录和翻译后(1)。应激反应中,HSP27表达增加了数倍以实现细胞对不利环境的抵抗。HSP27被MAPKAPK-2在Ser15、Ser78和Ser82磷酸化,作为p38 MAP激酶途径活化的结果(2,3)。HSP27磷酸化导致其四级结构改变,以至于从大分子量的同型多聚体变为二聚体和单体(4)。目前显示HSP27磷酸化和浓度增加可以调节肌动蛋白多聚化和再组织(5,6)。

  1. Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373.
  2. Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803.
  3. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  4. Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956.
  5. Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214.
  6. Rousseau, S. et al. (1997) Oncogene 15, 2169-2177.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications