Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-HSP27 (Ser82) Antibody #2401

heat shock   heat shock protein   hsp   hsp 27   hsp27   phospho-hsp  

No. Size Price
2401L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
2401S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2401 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 27 Rabbit
IHC-P 1:50
IF-IC 1:25
IHC-F 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), IHC-F=Immunohistochemistry (Frozen),

Specificity / Sensitivity

Phospho-HSP27 (Ser82) Antibody detects endogenous HSP27 only when phosphorylated at serine 82. The antibody does not recognize other heat shock proteins.

Phospho-HSP27 (Ser82) Antibody识别内源性的Ser82磷酸化的HSP27蛋白。此抗体不与其它HSF蛋白相互作用。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phospho- peptide corresponding to residues surrounding Ser82 of human HSP27. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, using Phospho-HSP27 (Ser82) Antibody (upper) or control HSP27 antibody (lower). HeLa cells were incubated at 42ºC for 0-2 hours as indicated.

对HeLa细胞抽提液,使用Phospho-HSP27 (Ser82) Antibody(上图)或对照HSP27 antibody(下图)进行Western blot分析。Hela细胞按要求在42℃中孵育0-2小时。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (A,C) or lambda phosphatase-treated (B,D), using Phospho-HSP27 (Ser82) Antibody (A,B) or HSP27 (G31) Monoclonal Antibody #2402 (C,D).

对石蜡包埋的人乳腺癌,对照(A,C)或用γ磷酸酶处理(B,D),使用Phospho-HSP27 (Ser82) Antibody (A,B)或HSP27 (G31) Monoclonal Antibody #2402 (C,D)进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-HSP-27 (Ser82) Antibody in the presence of control peptide (left) or antigen specific peptide (right).

对石蜡包埋的人肺癌使用Phospho-HSP-27 (Ser82) Antibody进行免疫组织化学分析,其中左图为对照肽,右图为抗原特异肽。

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen H1650 xenograft, showing cytoplasmic localization using Phospho-HSP27 (Ser82) Rabbit mAb.

对冰冻H1650异种移植物使用Phospho-HSP27 (Ser82) Rabbit mAb兔单抗进行免疫组化分析,显示出胞质定位。



Confocal immunofluorescent analysis of HeLa cells, anisomycin-treated (left) or untreated (right), using Phospho-HSP27 (Ser82) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

对HeLa细胞,加入anisomycin处理(左)或未处理(右),使用Phospho-HSP27 (Ser82) Antibody(绿色)进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor®555 phalloidin(红色)标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts of various cell lines, using Phospho-HSP27 (Ser82) Antibody #2401 (upper) or control HSP27 Antibody #2402 (lower).

对多个细胞抽提液,使用Phospho-HSP27 (Ser82) Antibody #2401(上图)或对照HSP27 Antibody #2402 (下图)进行Western blot分析。


Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78 and Ser82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).

热休克蛋白(HSP)27是小HSP之一,在多种细胞和组织中都有不同水平的组成性表达。像其它小HSP一样,HSP27的调节也发生在转录和翻译后(1)。应激反应中,HSP27表达增加了数倍以实现细胞对不利环境的抵抗。HSP27被MAPKAPK-2在Ser15、Ser78和Ser82磷酸化,作为p38 MAP激酶途径活化的结果(2,3)。HSP27磷酸化导致其四级结构改变,以至于从大分子量的同型多聚体变为二聚体和单体(4)。目前显示HSP27磷酸化和浓度增加可以调节肌动蛋白多聚化和再组织(5,6)。

  1. Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373.
  2. Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803.
  3. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  4. Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956.
  5. Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214.
  6. Rousseau, S. et al. (1997) Oncogene 15, 2169-2177.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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