Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Na,K-ATPase α1 (D4Y7E) Rabbit mAb #23565

sc-21712   sc-28800  

No. Size Price
23565S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
23565 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 100 Rabbit IgG
IHC-P 1:250
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Na,K-ATPase α1 (D4Y7E) Rabbit mAb recognizes endogenous levels of total Na,K-ATPase α1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro12 of human Na,K-ATPase α1 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of SK-MEL-28 (left), SNB19 (middle) or A-204 (right) cells using Na,K-ATPase α1 (D4Y7E) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-MEL-28, SNB19 and A-204 cells using Na,K-ATPase α1 (D4Y7E) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Na,K-ATPase α1 (D4Y7E) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human appendix using Na,K-ATPase α1 (D4Y7E) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded SK-MEL-28 (left) and A-204 (right) cell pellets using Na,K-ATPase α1 (D4Y7E) Rabbit mAb.

Background

The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic α subunit and a β subunit (reviewed in 1). Several phosphorylation sites have been identified for the α1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase (2), Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA (3-5). All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule (6). Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the α subunit (7). Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors (8,9).

  1. Therien, A.G. and Blostein, R. (2000) Am J Physiol Cell Physiol 279, C541-66.
  2. Féraille, E. et al. (1999) Mol Biol Cell 10, 2847-59.
  3. Fisone, G. et al. (1994) J Biol Chem 269, 9368-73.
  4. Feschenko, M.S. and Sweadner, K.J. (1995) J Biol Chem 270, 14072-7.
  5. Beguin, P. et al. (1994) J Biol Chem 269, 24437-45.
  6. Yingst, D.R. et al. (2004) Am J Physiol Renal Physiol 287, F713-21.
  7. Al-Khalili, L. et al. (2004) J Biol Chem 279, 25211-8.
  8. Tian, J. et al. (2006) Mol Biol Cell 17, 317-26.
  9. Liang, M. et al. (2006) J Biol Chem 281, 19709-19.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications