Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-Threonine-X-Arginine Antibody #2351

general   motif   PBS   phosphothreonine   substrate   TXR  

No. Size Price
2351S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2351 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,All Species Expected, Endogenous Rabbit
IHC-P 1:800
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-Threonine-X-Arginine Antibody detects endogenous levels of proteins containing the phospho-Thr-X-Arg motif. This antibody detects phosphorylated Thr followed by Arg or Lys at the +2 position, though its reactivity is lower for Lys compared to Arg at the +2 position. The antibody does not cross-react with nonphospho-Thr or phospho-Ser in the same motif. It recognizes phospho-Thr in the FFT*R motif in PKCbeta II but does not recognize phospho-Thr in other motifs that lack Lys or Arg at +2. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-Threonine-X-Arginine Antibody识别内源性的包含磷酸化Thr-X-Arg基序的蛋白。此抗体识别+2位是精氨酸或赖氨酸的磷酸化苏氨酸,对+2位是赖氨酸比+2位是精氨酸的识别能力稍弱。此抗体与包含非磷酸化苏氨酸和非磷酸化丝氨酸的相同基序没有交叉反应。其识别PKCβ中FFT*R模体里的磷酸化苏氨酸,但不识别其它缺乏+2位赖氨酸或精氨酸模体中的磷酸化苏氨酸。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide containing the phospho-Thr-X-Arg motif. Antibodies are purified by protein A and peptide affinity chromatography.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing staining of proteins containing phosphorylated threonine-X-arginine motifs, using Phospho-Threonine-X-Arginine Antibody.

对石蜡包埋的人乳腺癌使用Phospho-Threonine-X-Arginine Antibody进行免疫组化分析,显示包含苏氨酸-X-精氨酸模体的蛋白染色。



Phospho-Threonine-X-Arginine Antibody ELISA: Signal-to-noise ratio of phospho- versus nonphospho-peptides containing the phospho-threonine-X-arginine motif. (T* denotes phosphorylated threonine.)

Phospho-Threonine-X-Arginine Antibody ELISA分析:包含苏氨酸-X-精氨酸模体的磷酸化和非磷酸化的信噪比。(T*代表磷酸化的苏氨酸)

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated, TPA-treated or calyculin A-treated, using Phospho-Threonine-X-Arginine Antibody.

对Jurkat细胞,未处理或TPA或calyculin A处理,使用Phospho-Threonine-X-Arginine Antibody进行Western blot分析。


Some signaling molecules can be regulated by phosphorylation at a specific threonine followed by arginine or lysine at the +2 position. For example, conventional PKC isozymes phosphorylate substrates containing serine or threonine with Arg or Lys at the -3, -2 and +2 positions (1-2). c-Raf, a mitogen-activated protein kinase and the main effector recruited by GTP-bound Ras, is phosphorylated at Thr481 and Thr491 followed by Lys at the +2 position (3). Phosphorylation of these sites is important for enzyme activities. To determine the phosphorylation state of Thr in the Thr-X-Arg motif, and to identify potential new phosphorylation sites with this motif, Cell Signaling Technology has developed a Phospho-Threonine X-Arginine Antibody that recognizes phosphorylated Thr followed by Arg or Lys at the +2 position.

一些信号分子能被特异性的+2位是精氨酸或赖氨酸的苏氨酸磷酸化所调节。例如,传统型PKC同工酶磷酸化含丝氨酸或苏氨酸的底物,要求精氨酸或赖氨酸在-3、-2和+2位(1-2)。c-Raf是一个丝裂原活化的蛋白激酶,是GTP结合的RAS招募的主要效应物,磷酸化修饰+2位是赖氨酸的Thr481和Thr491位点(3)。这些位点的磷酸化是酶活性调节所需。为了确定Thr-X-Arg基序中苏氨酸的磷酸化状态以及鉴别新的潜在的此基序中的磷酸化位点,CST开发了Phospho-Threonine X-Arginine Antibody,可以识别+2位有精氨酸或赖氨酸的磷酸化苏氨酸。

  1. Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60.
  2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
  3. Zhang, B.H. and Guan, K.L. (2000) EMBO J 19, 5429-39.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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