Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb #2338

MAPKK 1   MAPKK 2  

No. Size Price
2338L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
2338S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2338 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human,Mouse,Rat,Monkey, Endogenous 45 Rabbit IgG
IHC-P 1:50
F 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry,

Homology

Species predicted to react based on 100% sequence homology: Dog,

Specificity / Sensitivity

Phospho-MEK1/2 (Ser 221) (166F8) Rabbit mAb detects endogenous levels of MEK1/2 only when phosphorylated at serine 221.

Phospho-MEK1/2 (Ser 221) (166F8) Rabbit mAb兔单抗能够检测内源性的丝氨酸(221位点)磷酸化的MEK1/2蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser221 of human MEK1/2.

该单克隆抗体是采用合成的与人源 MEK1/2蛋白丝氨酸(221位点)周围相一致的磷酸化肽段免疫动物而产生的。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb.

使用Phospho-MEK1/2 (Ser221)(166F8) Rabbit mAb兔单抗,对石蜡包埋的人乳腺癌组织进行免疫组织化学检测。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded prostate carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb.

使用Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb兔单抗,对未处理的(左)或用lambda磷酸酶处理的(右)的石蜡包埋的人前列腺癌组织进行免疫组织化学检测。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-MEK1/2 (Ser221)(166F8) Rabbit mAb.

使用Phospho-MEK1/2 (Ser221)(166F8) Rabbit mAb兔单抗,对石蜡包埋的人乳腺癌组织进行免疫组织化学方法检测。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or TPA and UV-treated, using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb (upper) or MEK1/2 Antibody #9122 (lower).

使用Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb兔单抗 (上图)和MEK1/2 Antibody兔多抗 #9122 (下图)对未处理的、TPA加紫外照射的HeLa、NIH/3T3细胞的提取物进行Western blot检测。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or PMA-treated (green), using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb compared to a nonspecific negative control antibody (red).

使用Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb兔单抗,对未处理(蓝色)和PMA处理(绿色)的Jurkat细胞进行流式细胞检测。红色为使用非特异的阴性对照抗体的结果。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of formalin fixed, paraffin-embedded A375 cells, untreated (left) or treated with Raf1 Kinase Inhibitor 1 (right), using Phospho-MEK1/2 (Ser 221) (166F8) Rabbit mAb.

使用Phospho-MEK1/2 (Ser 221) (166F8) Rabbit mAb兔单抗,对未处理的(左)或用Raf1 Kinase Inhibitor 1处理的(右)的福尔马林固定 、石蜡包埋的A375细胞进行免疫组织化学检测。

Background

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

MEK1、MEK2,也被称为MAPK或Erk激酶,它们是双特异性蛋白激酶,在丝裂原活化蛋白激酶级联反应中发挥作用,控制细胞生长和分化(1-3)。MEK1、MEK2的活化是通过Raf样分子对两个丝氨酸(217 /221位点)的磷酸化而实现的,这两个丝氨酸位点位于亚结构域VIII的活化环内。多种生长因子和细胞因子以及膜去极化和钙内流都能激活MEK1/2(1-4)。组成型活化的MEK1/2能够诱导NIH/3T3细胞的转化和PC-12细胞的分化(4)。MEK蛋白通过将p44、p42 MAPK激酶上位于亚结构域VIII的活化环内的丝氨酸和苏氨酸磷酸化,激活p44、 p42 MAPK激酶。

  1. Crews, C.M. et al. (1992) Science 258, 478-480.
  2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-19.
  3. Rosen, L.B. et al. (1994) Neuron 12, 1207-21.
  4. Cowley, S. et al. (1994) Cell 77, 841-52.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications