Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325

CDK   general   MAPK   motif   PBS   PXSP   SPXK   SPXR   substrate  

No. Size Price
2325S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2325 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,All Species Expected, Endogenous Rabbit IgG
IP 1:50
IHC-P 1:200
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb detects phospho-serine in a PXS*P or S*PXR/K motif, as well as a PXS*PXR/K motif. The antibody is phospho-specific, and does not react with phospho-threonine- or phospho-tyrosine-containing peptides/proteins. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb兔单抗识别PXS*P或S*PXR/K基序,以及PXS*PXR/K基序的磷酸化丝氨酸。此抗体是磷酸化特异性的,与含磷酸化苏氨酸或磷酸化酪氨酸的肽段或蛋白无交叉反应。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-MAPK/CDK substrate peptides .

该单克隆抗体用合成的磷酸化MAPK/CDK底物多肽免疫动物制备。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved A431 cells, untreated or treated in culture with EGF, and HeLa cells, untreated, hydroxyurea-treated (G1/S) or Nocodazole-treated (G2/M), using Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb.

对血清饥饿处理的A431细胞抽提液,未处理或EGF培养,以及Hela细胞,未处理或羟基脲处理(G1/S)或诺考达唑处理(G2/M),使用Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb进行Western blot分析。

ELISA-Peptide

ELISA-Peptide

Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (S* denotes phosphorylated serine.)

Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb ELISA分析:磷酸化和非磷酸化的信噪比。(S* 代表磷酸化的丝氨酸)

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb.

对石蜡包埋的人结肠癌,使用Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb.

对石蜡包埋的人肺癌,使用Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or lambda phosphatase-treated (right), using Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb.

对石蜡包埋的人乳腺癌对照(左)和λ磷酸酶处理(右),使用Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb进行免疫组化分析。

Background

The MAPK and CDK families of serine/threonine protein kinases play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-3). MAPK phosphorylates substrates with the consensus sequence PX(S/T)P, and CDKs phosphorylate substrates containing the consensus sequence (S/T)PXK/R. Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline, motifs PXS*/T*P and/or S*PXK/R, for use in the study and discovery of new MAPK and CDK substrates (4,5).

MAPK和CDK丝氨酸苏氨酸蛋白激酶家族在细胞增殖和周期调控中扮演重要角色。这些激酶磷酸化脯氨酸之前的苏氨酸或丝氨酸(1-3)。MAPK磷酸化含有一致序列PX(S/T)P的底物,CDK磷酸化含有一致序列(S/T)PXK/R的底物。CST公司开发了可以结合脯氨酸之前的磷酸化苏氨酸、基序PXS*/T*P和、或S*PXK/R的抗体,用以研究和发现新的MAPK和CDK底物(4,5)。

  1. Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
  2. Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.
  3. Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
  4. Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6.
  5. Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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