Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Synapsin (Ser9) Antibody #2311

Synapsin  

No. Size Price
2311S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2311 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 77 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Synapsin (Ser9) Antibody detects endogenous levels of synapsin only when phosphorylated at serine 9. The antibody is expected to recognize phosphorylated serine 9 of all synapsin isoforms.

Phospho-Synapsin (Ser9) Antibody 兔多抗识别内源性的Ser9磷酸化的synapsin蛋白。该抗体可以识别所有Ser9磷酸化的synapsin亚型。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serine 9 of human synapsin I. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体由合成的人类synapsin I蛋白Ser9位点附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of mouse brain homogenates, untreated or phosphatase-treated, using Phospho-Synapsin (Ser9) Antibody (upper) or Synapsin Antibody #2312 (lower).

Western blot分析小鼠大脑匀浆,未处理或磷酸酶处理,使用的抗体是Phospho-Synapsin (Ser9) Antibody 兔多抗(上图)或Synapsin Antibody 兔多抗#2312(下图)。

Background

Synapsins, a group of at least five related members (synapsins Ia, Ib, IIa, IIb, and IIIa), are abundant brain proteins essential for regulating neurotransmitter release (1,2). All synapsins contain a short amino-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I (1). Phosphorylation of the synapsin amino-terminal domain at Ser9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles (2).

Synapsins是一组至少包括五个相关成员(Synapsins IA,IB,IIA,IIB,和IIIa)的蛋白,在脑内丰富,在调节神经递质的释放中必不可少(1,2)。所有的synapsins都包含一个短的氨基末端结构域,它高度保守,可被PKA或钙调蛋白激酶I磷酸化(1)。synapsin氨基末端域Ser9的磷酸化会抑制其与磷脂的结合,将其从突触囊泡上分解出来(2)。

  1. Greengard, P. (1987) Mol Neurobiol 1, 81-119.
  2. Hosaka, M. et al. (1999) Neuron 24, 377-87.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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