Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-DARPP-32 (Thr75) Antibody #2301

DARPP   DARPP-32   darpp32  

No. Size Price
2301S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2301 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse,Rat, Endogenous 32 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-DARPP-32 (Thr75) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 75. The antibody does not cross-react with DARPP-32 phosphorylated at Thr34.

Phospho-DARPP-32 (Thr75) Antibody 兔多抗识别内源性的Thr75磷酸化的DARPP-32蛋白。此抗体与Thr34磷酸化的DARPP-32蛋白没有交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr75 of human DARPP-32. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from rat brain cortex, untreated or phosphorylated in vitro by PKA, MAP kinase or cdc2 kinase, using Phospho-DARPP-32 (Thr75) Antibody #2301 (top), Phospho-DARPP-32 (Thr34) Antibody (middle) or DARPP-32 Antibody #2302 (bottom).

Western blot分析大鼠大脑皮层(未处理,或PKA、MAP激酶和cdc2激酶体内磷酸化处理),使用的抗体是Phospho-DARPP-32 (Thr75) Antibody 兔多抗(上图),Phospho-DARPP-32 (Thr34) Antibody 兔多抗(中图)或DARPP-32 Antibody 兔多抗#2302(下图)。


DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological and behavioral phenotype (3).

DARPP-32(多巴胺和环磷酸腺苷调节的磷蛋白,相对分子质量32000)是一种在纹状体中型棘神经元高度富集的胞浆蛋白(1)。这是一个双功能的信号分子,控制丝氨酸/苏氨酸激酶和丝氨酸/苏氨酸磷酸酶的活性(2)。多巴胺通过刺激多巴胺D1受体和激活PKA来磷酸化DARPP-32。PKA磷酸化DARPP-32 Thr34,将其转换成一种蛋白磷酸酶1抑制剂(1)。DARPP-32被细胞周期蛋白依赖性激酶5(CDK5)在Thr75磷酸化后,被转换成PKA的抑制剂(2)。针对性的DARPP-32基因删除的小鼠表现出生化,电生理和行为的改变(3)。

  1. Nishi, A. et al. (1997) J. Neurosci. 17, 8147-8155.
  2. Bibb, J.A. et al. (1999) Nature 402, 669-671.
  3. Fienberg, A.A. et al. (1998) Science 281, 838-842.

Application References

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