Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-(Ser) PKC Substrate Antibody #2261

general   KXSXK   KXSXR   motif   PBS   phospho serine   phospho-serine   phosphoserine   PKC   R/KXSXR/K   RXSXK   RXSXR   substrate  

No. Size Price
2261L 600 µl ( 60 western blots ) ¥8,792.00 现货查询 购买询价
2261S 200 µl ( 20 western blots ) ¥3,900.00 现货查询 购买询价
2261 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Rabbit
IP 1:25
E-P 1:500

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-(Ser) PKC Substrate Antibody detects endogenous levels of many cellular proteins only when phosphorylated at serine residues surrounded by Arg or Lys at the -2 and +2 positions and a hydrophobic residue at the +1 position. The antibody does not cross-react with nonphosphorylated serine residues, with phospho-threonine in the same motif, or with phospho-serine in other motifs. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-(Ser) PKC Substrate Antibody可识别内源性在+2和-2位上有精氨酸或赖氨酸,并且+1位是疏水氨基酸的磷酸化丝氨酸的细胞内蛋白质。该抗体不与非磷酸化丝氨酸残基、同样基序中磷酸化的苏氨酸残基、或其它基序中的磷酸化丝氨酸残基发生交叉反应。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及相应国外专利)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phospho-PKC substrate peptides . Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or treated with PMA (50 ng/ml) and ionomycin (1 µM for 20 minutes prior to lysis) and subjected to 2-D electrophoresis, using Phospho-(Ser) PKC Substrate Antibody.

对未处理或50ng/ml PMA和1uM ionomycin裂解前处理20分钟的Jurkat细胞抽提液进行二维电泳,使用Phospho-(Ser) PKC Substrate Antibody进行Western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from WEHI 231 cells and Jurkat cells, untreated or TPA-treated, using Phospho-(Ser) PKC Substrate Antibody.

对未处理或TPA处理的WEHI 231细胞和Jurkat细胞抽提液使用Phospho-(Ser) PKC Substrate Antibody进行Western blot分析。



Phospho-(Ser) PKC Substrate Antibody ELISA assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (S* denotes phosphorylated serine.)

Phospho-(Ser) PKC Substrate Antibody ELISA分析:磷酸化和非磷酸化的信噪比。(S* 代表磷酸化的丝氨酸)


Although protein kinase C (PKC) family members are involved in a number of signal transduction processes including secretion, gene expression, proliferation, and muscle contraction (1,2), many of their substrates remain unidentified. Isozymes of PKC are subdivided into conventional PKCs (cPKC), novel PKCs (nPKC), and atypical PKCs (aPKC). PKCα, βI, βII, and γ isoforms belong to the cPKC group (1). When activated, cPKC isozymes phosphorylate substrates containing Ser or Thr, with Arg or Lys at the -3, -2, and +2 positions, and a hydrophobic amino acid at position +1 (1-3). To identify potential PKC substrates, Cell Signaling Technology has developed antibodies that recognize this motif. Phospho-(Ser) PKC Substrate (D13C5/D47H10/D50E3) Rabbit mAb is specific to cPKC substrates containing phospho-Ser.

尽管蛋白激酶C(PKC)家族成员参与多种信号转导过程,包括分泌、基因表达、增殖和肌肉收缩(1,2),但它们的很多底物仍然未确定。PKC的同工酶分为传统型(cPKC),新型PKC(nPKC)和非典型PKC(aPKC)。PKCα, βI, βII, 和γ属于cPKC组(1)。激活后cPKC同工酶磷酸化精氨酸或赖氨酸在-3,-2和+2位,和+1位是疏水氨基酸的丝氨酸和苏氨酸(1-3)。为了鉴别潜在的PKC底物,Cell Signaling Technology公司开发了识别此基序的抗体。Phospho-(Ser) PKC Substrate (D13C5/D47H10/D50E3) Rabbit mAb兔单抗特异性识别包含磷酸化丝氨酸的cPKC底物。

  1. Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60.
  2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
  3. Obata, T. et al. (2000) J Biol Chem 275, 36108-15.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

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