Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-HER2/ErbB2 (Tyr1221/1222) Antibody #2249

c-erb B2/neu protein   CD340 antigen   erbB-2   HER2   herstatin   v-erb-b2 erythroblastic leukemia viral oncogene homolog 2. neuro/glioblastoma derived oncogene homolog (avian)  

No. Size Price
2249S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2249 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 185 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-HER2/ErbB2 (Tyr1221/1222) Antibody detects endogenous levels of ErbB2 only when phosphorylated at tyrosine 1221/1222. The antibody may detect other activated Erb family members.

磷酸化HER2/ErbB2 (Tyr1221/1222)抗体仅在Tyr 1221/1222被磷酸化后才能检测到内源性ErbB2的存在。本抗体可能与其它激活的ErbB家族蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding tyrosine 1221/1222 of human ErbB2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体通过用多肽免疫动物得到,该磷酸化多肽是根据人的ErbB2蛋白Tyr 1221/1222附近的氨基酸序列合成的。抗体经过protein A和亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated SKBR3 and MCF-7 cells, untreated or treated with neuregulin (NRG), using Phospho-HER2/ErbB2 (Tyr1221/1222) Antibody (upper) and HER2/ErbB2 Antibody #2242 (lower). Western blot法检测下列细胞提取物:未经处理的SKBR3 细胞,未经处理的MCF7 细胞,经过neuregulin (NRG) 处理的MCF7 细胞。使用抗体为:Phospho-HER2/ErbB2 (Tyr1221/1222) Antibody (上) 和 HER2/ErbB2 Antibody #2242 (下)。

Background

The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5).

原癌基因ErbB2 (HER2)编码185kDa的受体样跨膜糖蛋白,该蛋白具有固有的酪氨酸激酶的活性 (1)。ErbB2的配体目前尚不清楚,但在高表达的ErbB2与其它ErbB家族成员形成异源二聚体时,即使缺少配体,其酶活性仍能被激活(2)。将近40%的人类乳腺癌中能够检测到ErbB2基因的扩增及其产物的过量表达。泛素连接酶c-Cbl结合在ErbB2的Tyr1112上能够导致ErbB2聚泛素化并促进其降解(4)。ErbB2是乳腺癌等多种癌肿的关键治疗靶点,通过c-Cbl介导的蛋白水解途径靶向调控ErbB2的降解是一种潜在的治疗手段。磷酸化ErbB2的激酶位点Tyr877(同源于pp60c-Src 的Tyr416)能调控ErbB2的生物活性。ErbB2的自磷酸化位点是Tyr1248和 Tyr1221/1222,这些位点的磷酸化将ErbB2与Ras-Raf-MAP激酶的信号转导途径联系起来(1,5)。

  1. Muthuswamy, S.K. et al. (1999) Mol Cell Biol 19, 6845-57.
  2. Qian, X. et al. (1994) Proc Natl Acad Sci USA 91, 1500-4.
  3. Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4.
  4. Klapper, L.N. et al. (2000) Cancer Res 60, 3384-8.
  5. Kwon, Y.K. et al. (1997) J Neurosci 17, 8293-9.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications