Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-S6 Ribosomal Protein (Ser235/236) Antibody #2211

ps6   ribosomal   ribosomal protein   ribosome   rps6  

No. Size Price
2211L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
2211S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2211 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,S. cerevisiae, Endogenous 32 Rabbit
IP 1:100
IHC-P 1:400
F 1:100
IF-IC 1:100
IHC-F 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-F=Immunohistochemistry (Frozen),

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus,

Specificity / Sensitivity

Phospho-S6 Ribosomal Protein (Ser235/236) Antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at serine 235 and 236. This antibody does not detect ribosomal protein S6 phosphorylated at other sites.

Phospho-S6 Ribosomal Protein (Ser235/236) Antibody检测仅在serine 235和236位点磷酸化的内源性核糖体S6蛋白。该抗体不能检测其它位点磷酸化的核糖体S6蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6. Antibodies are purified by protein A and peptide affinity chromatography.

通过人工合成人源核糖体S6蛋白Ser235和Ser236位点周围序列相应的磷酸化片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untreated or treated with 20% FBS for the indiccated time, using , Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (upper) or Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 (lower).

使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (上图)和Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 (下图),免疫印迹(Western blot)分析293细胞,分为对照组和20% FBS处理组(如图中所示的时间)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical staining of paraffin-embedded human melanoma, showing cytoplasmic localization of phosphorylated S6 ribosomal protein, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.

使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody,免疫组化分析人类黑色素瘤组织石蜡切片,结果显示磷酸化的S6核糖体蛋白定位在细胞质。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody preincubated with control peptide (left) or Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (right).

使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody,免疫组化分析人类乳腺癌组织石蜡切片,其分别孵育对照多肽(左图)和Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (右图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or calf-intestinal phosphatase (CIP) treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.

使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody,免疫组化分析人类乳腺癌组织石蜡切片,分为对照组(左图)和牛小肠磷酸酶(CIP)处理组(右图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.

使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody,免疫组化分析LNCaP细胞石蜡切片,分为对照组(左图)和rapamycin处理组(右图)。

IHC-P (paraffin)

IHC-P (paraffin)

Imunohistochemical analysis of paraffin-embedded human ovarian carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.

使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody,免疫组化分析卵巢癌组织石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.

使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody,免疫组化分析人类肾脏细胞癌石蜡切片。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH/3T3 cells, U0126- and Rapamycin-treated (blue) or serum-treated (green), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody compared to a nonspecific negative control antibody (red).

与非特异阴性对照抗体(红色),使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody标记,流式细胞术分析NIH/3T3细胞,细胞分为U0126和Rapamycin处理组(蓝色)和血清处理组(绿色)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.

使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody,免疫组化分析人类肺癌组织石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.

使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody,免疫组化分析人类结肠癌组织石蜡切片。

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen U87MG xenograft, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.

使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody,免疫组化分析U87MG异种移植物组织的冰冻切片。

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells, either U0126/LY294002/Rapamycin-treated (left) or insulin-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

生长因子和有丝分裂原有效促进维持细胞生长和增殖的一个方面就是通过上调mRNA翻译(1,2)。生长因子和有丝分裂原能诱导p70 S6激酶的激活和随后S6核糖体蛋白的磷酸化。S6核糖体蛋白的磷酸化与mRNA转录本的翻译增加有关,而这个mRNA转录本是在5端非翻译区含有一个寡嘧啶区(2)。这些特殊的mRNA转录本 (5'TOP) 能够编码涉及细胞周期进程的蛋白质如翻译必须的核糖体蛋白和延伸因子(2,3)。重要的S6核糖体蛋白的磷酸化位点包含数个定位在S6蛋白一个小的羧基末端区域残基(Ser235, Ser236, Ser240, and Ser244) (4,5)。

  1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
  2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
  3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
  4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
  5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.

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