Cell Signaling Technology

Product Pathways - Protein Stability

Bortezomib #2204

boronic acid dipeptide   NF-kB   p53   reversable proteasome inhibitor   ubiquitin  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Molecular Characterization

C19H25BN4O4

Purity

99%

Structure

Structure

Chemical structure of Bortezomib.硼替佐米的化学结构。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or treated (+) with Bortezomib (100 nM, 6 hr), using Ubiquitin Antibody #3933 (left), p53 (1C12) Mouse mAb #2524 (middle), and β-Actin (13E5) Rabbit mAb #4970 (right).Western blot分析NIH/3T3细胞提取物,未处理(-)或用硼替佐米(100 nM, 6小时,+)处理,使用的抗体是:Ubiquitin Antibody #3933(左图),p53 (1C12) Mouse mAb #2524(中间),β-Actin (13E5) Rabbit mAb #4970(右图)。

Western blot analysis of L363 cells, untreated (-) or treated (+) with Bortezomib (24 hr) as indicated, using K48-linkage Specific Polyubiquitin Antibody #4289.Western blot分析L363细胞,未处理(-)或用硼替佐米(24小时,+)按照指示处理,使用的抗体是:K48-linkage Specific Polyubiquitin Antibody #4289。

Directions for Use

Bortezomib is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 2.5 mg in 6.51 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-1000 nM for 2-48 hours. Soluble in DMSO and ethanol at 200 mg/ml, very poorly soluble in plain water.

提供的硼替佐米为冻干粉。对于1mM浓度贮存液,在2.5mg冻干粉中加入6.51ml DMSO。工作浓度和处理时间根据所需的预期效果来确定,一般是1-1000nM,处理2-48小时。能溶于DMSO和乙醇,200 mg/ml,很难溶于普通的水。

Background

Bortezomib (C19H25BN4O4), a boronic acid dipeptide, is a specific, potent, and reversable proteasome inhibitor that has also been shown to have a stabilizing effect on proteins that inhibit cell survival and cell cycle progression, such as p53 (1,2). Researchers have demostrated that bortezomib inhibits activation and nuclear translocation of NF-κB, subsequently decreasing early tumor survival (3). The increase in bortezomib-stablilized, misfolded, and ubiquitinated proteins that interfere with cell survival and other important pathways further support its anti-tumor effects (3).

硼替佐米(C19H25BN4O4)——硼酸二肽,是一种特异的、有效的、可逆的蛋白酶抑制剂,对抑制细胞存活和细胞周期进程的蛋白质有一个稳定效应,如p53(1,2)。研究者发现硼替佐米能抑制NF-κB的活化和核转运,从而减少早期肿瘤存活(3)。能干扰细胞存活和其他重要通路的、硼替佐米稳定的、错误折叠的、泛素化的蛋白的增加能进一步加强它的抗肿瘤作用(3)。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications