Cell Signaling Technology

Product Pathways - Protein Stability

Bortezomib #2204

boronic acid dipeptide   NF-kB   p53   reversable proteasome inhibitor   ubiquitin  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Molecular Characterization






Chemical structure of Bortezomib.硼替佐米的化学结构。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or treated (+) with Bortezomib (100 nM, 6 hr), using Ubiquitin Antibody #3933 (left), p53 (1C12) Mouse mAb #2524 (middle), and β-Actin (13E5) Rabbit mAb #4970 (right).Western blot分析NIH/3T3细胞提取物,未处理(-)或用硼替佐米(100 nM, 6小时,+)处理,使用的抗体是:Ubiquitin Antibody #3933(左图),p53 (1C12) Mouse mAb #2524(中间),β-Actin (13E5) Rabbit mAb #4970(右图)。

Western blot analysis of L363 cells, untreated (-) or treated (+) with Bortezomib (24 hr) as indicated, using K48-linkage Specific Polyubiquitin Antibody #4289.Western blot分析L363细胞,未处理(-)或用硼替佐米(24小时,+)按照指示处理,使用的抗体是:K48-linkage Specific Polyubiquitin Antibody #4289。

Directions for Use

Bortezomib is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 2.5 mg in 6.51 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-1000 nM for 2-48 hours. Soluble in DMSO and ethanol at 200 mg/ml, very poorly soluble in plain water.

提供的硼替佐米为冻干粉。对于1mM浓度贮存液,在2.5mg冻干粉中加入6.51ml DMSO。工作浓度和处理时间根据所需的预期效果来确定,一般是1-1000nM,处理2-48小时。能溶于DMSO和乙醇,200 mg/ml,很难溶于普通的水。


Bortezomib (C19H25BN4O4), a boronic acid dipeptide, is a specific, potent, and reversable proteasome inhibitor that has also been shown to have a stabilizing effect on proteins that inhibit cell survival and cell cycle progression, such as p53 (1,2). Researchers have demostrated that bortezomib inhibits activation and nuclear translocation of NF-κB, subsequently decreasing early tumor survival (3). The increase in bortezomib-stablilized, misfolded, and ubiquitinated proteins that interfere with cell survival and other important pathways further support its anti-tumor effects (3).


Application References

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