Cell Signaling Technology

Product Pathways - Apoptosis

Etoposide #2200

anti-tumor   apoptosis   cleaved parp   covalent topoisomerase II-cleaved DNA   VP-16  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Molecular Characterization






Chemical structure of etoposide.依托泊苷的化学结构。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated (-) or etoposide-treated (25 μM, overnight; +), using Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625 (upper) or total PARP Antibody #9542 (lower).Western blot分析Jurkat细胞提取物,未处理(-)或用依托泊苷(25 μM, 过夜; +)处理,使用的抗体是:Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625(上图)、total PARP Antibody #9542(下图)。

Directions for Use

Etoposide is supplied as a lyophilized powder. For a 50 mM stock, reconstitute the 5.9 mg in 200 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µM for 4-24 hr. Soluble in DMSO at 100 mg/ml; very poorly soluble in ethanol and water with maximum solubility in water ~20-50 µM.

提供的依托泊苷为冻干粉。对于50 mM浓度贮存液,在5.9mg冻干粉中加入200 µl DMSO。工作浓度和处理时间根据所需的预期效果来确定,一般是5-50 µM,处理4-24小时。能溶于DMSO(100 mg/ml),难溶于乙醇和水,在水中的最大溶解度约为20-50 µM。


An anti-tumor agent that is commonly used as an apoptosis inducer, etoposide (VP-16) is a topoisomerase II inhibitor with an IC50 of 59.2 µM (1). Etoposide stabilizes a covalent topoisomerase II-cleaved DNA intermediate complex in the catalytic cycle of the enzyme, leading to genomic instability and cell death (2,3). This mechanism of action has been shown to delay progression of the cell cycle through the late S and early G2 phase (4,5).

依托泊苷(VP-16)是一种抗肿瘤剂,常用作凋亡诱导物,它还是一种拓扑异构酶II抑制剂(IC50为59.2 µM)(1)。依托泊苷能稳定酶催化性循环中的一种共价的拓扑异构酶II裂解的DNA中间复合物,导致基因组不稳定性和细胞死亡(2,3)。这种作用机制能通过S晚期和G2早期来延误细胞周期的进展(4,5)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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