Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

ESET (C1C12) Rabbit mAb #2196

ERG-associated protein with SET domain   H3-K9-HMTase 4   Histone H3-K9 methyltransferase 4   histone methyltransferase   HMT   SET domain bifurcated 1   SETB1   SETDB1  

No. Size Price
2196S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2196T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
2196 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 180 Rabbit IgG
IP 1:50
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

ESET (C1C12) Rabbit mAb detects endogenous levels of total ESET protein. The antibody does not cross-react with other SET-domain containing histone methyltransferase proteins.

ESET (C1C12) Rabbit mAb兔单抗能够检测内源性ESET总蛋白水平。该抗体不与包含SET区域的组蛋白甲基转移酶发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human ESET protein.

通过合成的与人源ESET蛋白羧基端相应多肽片段去免疫动物从而制备出此单克隆抗体。

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells using ESET (C1C12) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

使用ESET (C1C12) Rabbit mAb (绿色),共聚焦免疫荧光分析MCF-7细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。

Western Blotting

Western Blotting

Western blot analysis of cell lysates from MCF7 and 293 cells using ESET (C1C12) Rabbit mAb.

使用ESET (C1C12) Rabbit mAb,免疫印迹(Western blot)分析MCF7和293细胞中ESET (C1C12)的蛋白水平。

Background

The Erg-associated protein with SET domain (ESET), also known as SET-domain, bifurcated 1 (SETDB1) protein, is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). ESET also contains tudor and methyl-CpG-binding domains, which may coordinate binding to methylated histones and methylated DNA, respectively (1). ESET methylates histone H3 Lys9, creating a transcriptionally repressive mark that facilitates gene silencing (1-3). However, unlike SUV39H histone H3 Lys9 methyltransferases, which function mainly in heterochromatin regions such as pericentric heterochromatin, ESET functions mainly in euchromatic regions to repress gene promoters (3). ESET interacts with a variety of proteins, including transcription factors (ERG), histone deacetylases (HDAC1/2), DNA methyltransferases (DNMT3A/B) and transcriptional co-repressors (mSin3A/B, MBD1, KAP-1, the ATFa-associated modulator mAM) (1-6). mAM forms a complex with ESET, stimulating its methyltransferase activity, specifically the conversion of di-methyl to tri-methyl histone H3 Lys9 (2). MBD1 recruits ESET to the CAF-1 complex to facilitate methylation of histone H3 Lys9 during replication-coupled chromatin assembly in S phase (5). DNMT3A recruits ESET to silenced promoters in cancer cells (7). ESET may play a role in the pathogenesis of Huntington's disease, since levels of ESET protein and tri-methyl histone H3 Lys9 are both increased in diseased brains (8).

Erg-associated protein with SET domain (ESET)也称为SET-domain bifurcated 1 (SETDB1) protein,它是一个组蛋白赖氨酸甲基转移酶家族成员,它们中每一个都包含一个保守催化的SET结构,该结构起初被鉴定在Drosophila Su[var]3-9、Enhancer of zeste和Trithorax蛋白中(1)。ESET也包含tudor和methyl-CpG-binding区域,这可以协调分别结合到甲基化组蛋白和甲基化的DNA(1)。ESET可使 histone H3蛋白Lys9位点甲基化,这增加一个有助于基因沉默的转录性抑制标记物(1-3)。然而,不像SUV39H histone H3 Lys9 甲基转移酶一样,该酶的功能主要在异染色质区域例如臂间异染色质(pericentric heterochromatin),ESET的功能主要在常染色质区域去抑制基因启动子(3)。ESET与多种蛋白质相互作用,包括转录因子(ERG)、组蛋白去乙酰酶 (HDAC1/2)、DNA甲基转移酶(DNMT3A/B) 和转录辅助抑制因子(mSin3A/B、MBD1、KAP-1、ATFa-associated modulator mAM) (1-6)。mAM形成一个ESET复合物,这能刺激它的甲基转移酶活性,特别是histone H3蛋白Lys9位点双甲基化转化到三甲基化(2)。在S期复制偶联染色质装配期间,MBD1招募ESET到CAF-1复合物从而有助于histone H3蛋白Lys9位点甲基化。在癌症细胞中DNMT3A蛋白招募ESET到沉默的启动子(7)。因为ESET蛋白水平和histone H3蛋白Lys9位点三甲基化都在病理大脑中表达增加,ESET可能在亨廷顿氏舞蹈病的发病机理上有重要作用(8)。

  1. Yang, L. et al. (2002) Oncogene 21, 148-152.
  2. Wang, H. et al. (2003) Mol. Cell 12, 475-487.
  3. Schultz, D.C. et al. (2002) Genes Dev. 16, 919-932.
  4. Yang, L. et al. (2003) Biochem. J. 369, 651-657.
  5. Sarraf, S.A. and Stancheva, I. (2004) Mol. Cell 15, 595-605.
  6. Ichimura, T. et al. (2005) J. Biol. Chem. 280, 13928-13935.
  7. Li, H. et al. (2006) J. Biol. Chem. 281, 19489-19500.
  8. Ryu, H. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 19176-19181.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

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