Cell Signaling Technology

Product Pathways - Protein Stability

MG-132 #2194

apoptosis Hep G2   calpain inhibitor   casein-degrading activity   MG132   Phospo-cyclin   proteasome inhibitor   ubiquitin-proteasome   Z-Leu-Leu-Leu-CHO   Z-LLL-al  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Molecular Characterization

C26H41N3O5

Purity

>98%

Structure

Structure

Chemical structure of MG-132.MG-132的化学结构。

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-1080 cells, untreated (-) or treated with MG-132 (10 μM, 4 hr; +), using Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb #3300.Western blot分析HT-1080细胞的提取物,未处理(-)或用MG-132 (10 μM, 4小时; +)处理,使用的抗体是:Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb #3300。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and HeLa cells, untreated (-) or treated with MG-132 (50 µM, 90 min; +), using Ubiquitin (P4D1) Mouse mAb #3936.Western blot分析293和HeLa细胞的提取物,未处理(-)或用MG-132 (50 µM, 90分钟; +)处理,使用的抗体是:Ubiquitin (P4D1) Mouse mAb #3936。

Directions for Use

MG-132 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 1 mg in 210.3 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µM for 1-24 hours. Soluble in DMSO or EtOH.

提供的MG-132为冻干粉。对于10 mM浓度贮存液,在1mg冻干粉中加入210.3 µl DMSO。工作浓度和处理时间根据所需的预期效果来确定,一般是5-50 µM,处理1-24小时。能溶于DMSO或乙醇。

Background

MG-132 is a potent proteasome and calpain inhibitor. Research studies have shown that MG-132 prevents degradation of the proteasome substrates SucLLVY-MCA and ZLLL-MCA with an IC50 of 850 nM and 100 nM, respectively, as well as inhibits casein-degrading activity of m-calpain with an IC50 of 1.25 µM (1). Investigators have demonstrated that MG-132 inhibits TNF-α-induced NF-κB activation and IL-8 release in A549 cells (2). Proteasome inhibition with MG-132 prevents degradation of short-lived proteins, which correlates with increased expression of HSP and ER chaperone proteins (3). MG-132 induces apoptosis in Hep G2 cells in a time- and dose-dependent manner (4). Proteasome inhibitors like MG-132 are important research tools for studying cellular degradation of the ubiquitin-proteasome pathway.

MG-132是一种有效的蛋白酶体和钙蛋白酶抑制剂。研究发现MG-132能阻止蛋白酶体底物SucLLVY-MCA(IC50为850nm)和ZLLL-MCA(IC50为100nm)的降解,也能抑制m-需钙蛋白酶(IC50为1.25 µM)的酪蛋白降解活性(1)。研究者已经证明MG-132能抑制TNF-α诱导的NF-κB活化和IL-8在A549细胞中的释放(2)。用MG-132进行的蛋白酶体抑制能阻止短效蛋白的降解,这些短效蛋白与表达增加的HSP和ER伴侣蛋白有关(3)。MG-132能通过一种时间和剂量依赖的方式诱导Hep G2细胞的凋亡(4)。蛋白酶体抑制剂,如MG-132,是研究泛素-蛋白酶体通路的细胞降解的重要工具。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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