Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Nocodazole #2190

ABL   anti-neoplastic   BRAF   c-KIT   MEK1   MEK2   MET   microtubule depolymerization   mitotic arrest   phospho histone   R 17934  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Molecular Characterization

C14H11N3O3S

Purity

>97%

Structure

Structure

Chemical structure of nocodazole.诺考达唑的化学结构。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells untreated (-) or treated with Nocodazole (0.1 μg/ml, 18 hr; +) or λ phosphatase (+), using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 (upper) or Histone H3 Antibody #9715 (lower).Western blot分析HeLa细胞提取物,未处理(-)或者用诺考达唑(0.1 μg/ml, 18小时; +)或λ磷酸酶(+)处理,使用的抗体是:Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377(上图)、Histone H3 Antibody #9715(下图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Nocodazole (0.1 μg/ml, 18 hr; +), using Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb #3520 (upper) or NPM Antibody #3542 (lower).Western blot分析HeLa细胞提取物,未处理(-)或用诺考达唑(0.1 μg/ml, 18小时; +)处理,使用的抗体是:Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb #3520(上图)、NPM Antibody #3542(下图)。

Directions for Use

Nocodazole is supplied as a lyophilized powder. For a 1 mg/ml stock, reconstitute the 10 mg in 10 ml DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-1 µg/ml for 12-48 hr. Soluble in DMSO.

提供的诺考达唑为冻干粉。对于1mg/ml浓度贮存液,在10 mg冻干粉中加入10 ml DMSO。工作浓度和处理时间根据所需的预期效果来确定,一般是0.1-1 µg/ml,处理12-48小时。能溶于DMSO。

Background

Nocodazole is an anti-neoplastic agent that reversibly interferes with the polymerization of microtubules (1). Widely-used as a cell cycle synchronizing agent in cell biology labs to induce mitotic arrest, investigators have demonstrated that high concentrations of nocodazole induce microtubule depolymerization, whereas low concentrations alter spindle microtubule dynamics, but microtubules do not depolymerize (2-4). Recent research studies have demonstrated nocodazole to be a common inhibitor of various cancer-related kinases, including: ABL, c-KIT, BRAF, MEK1, MEK2, and MET (5).

诺考达唑是一种抗肿瘤药物,能可逆地干扰微管的聚合(1)。它作为一种细胞周期同步化药物广泛用于细胞生物学实验室,能够诱导有丝分裂的停止,研究者已经证实高浓度的诺考达唑能诱导微管解聚,而低浓度时能改变纺锤体微管动力学,但微管不解聚(2-4)。最近的研究显示诺考达唑是一种常用的多种癌症相关激酶的抑制剂,包括ABL, c-KIT, BRAF, MEK1, MEK2和MET (5)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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