Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Phospho-CENP-A (Ser7) Antibody #2187

CENP-A   Centromere-autoantigen-A   Centromere-protein-A   h3   histone h3  

No. Size Price
2187S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2187 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 17 Rabbit
IP 1:25
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

Phospho-CENP-A (Ser7) Antibody detects endogenous levels of human CENP-A protein only when phosphorylated on Ser7. This antibody does not cross-react with other histone proteins, including Histone H3.

Phospho-CENP-A (Ser7) Antibody检测仅在Ser7位点磷酸化的内源性人源CENP-A蛋白水平。该抗体不能与其它组蛋白包括Histone H3发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser7 of human CENP-A protein. Antibodies are purified by peptide affinity chromatography.




Confocal immunofluorescent analysis of a mitotic HeLa cell using Phospho-CENP-A (Ser7) Antibody (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red). Phospho-CENP-A signal is localized to bright spots in the metaphase plate. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用Phospho-CENP-A (Ser7) Antibody (绿色)和β-Tubulin (9F3) Rabbit mAb 兔单抗(Alexa Fluor® 555 Conjugate) #2116 (红色)标记,共聚焦免疫荧光分析有丝分裂期间的HeLa细胞。Phospho-CENP-A信号定位在赤道板亮斑上。蓝色= DRAQ5® #4084 (DNA荧光染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells arrested in S phase or mitosis using Phospho-CENP-A (Ser7) Antibody (upper panel) or CENP-A Antibody #2186 (lower panel). S phase cells were treated for 12 hours with thymidine (2 mM), rinsed three times, released into normal growth medium for 10 hours and then treated an additional 12 hours with thymidine before harvesting. Mitotic cells were treated for 12 hours with thymidine, rinsed three times and then treated for 16 hours with paxitaxol (500 nM final).

使用Phospho-CENP-A (Ser7) Antibody (上图)或CENP-A Antibody #2186 (下图),免疫印迹(Western blot)分析停滞的S期或有丝分裂的HeLa细胞中Phospho-CENP-A (Ser7)和CENP-A蛋白水平。S期的细胞经过thymidine (2 mM)处理12小时,冲洗三次后,使用普通的细胞培养液培养10小时,然后在收集细胞之前用thymidine处理12小时。有丝分裂期细胞经过thymidine处理12小时,冲洗三次后,然后用paxitaxol处理 (最终浓度为500 nM )16小时。



Confocal immunofluorescent analysis of a mitotic HeLa cell using Phospho-CENP-A (Ser7) Antibody (green fluorescence, appearing as white in the composite image) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red). Phospho-CENP-A signal is localized to bright spots in the metaphase plate. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Modulation of chromatin structure plays a critical role in the regulation of transcription and replication of the eukaryotic genome. The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure (1). CENP-A, also known as the chromatin-associated protein CSE4 (capping-enzyme suppressor 4-p), is an essential histone H3 variant that replaces canonical histone H3 in centromeric heterochromatin (2). The greatest divergence between CENP-A and canonical histone H3 occurs in the amino-terminal tail of the protein, which binds linker DNA between nucleosomes and facilitates proper folding of centromeric heterochromatin (3). The amino-terminal tail of CENP-A is also required for recruitment of other centromeric proteins (CENP-C, hSMC1, hZW10), proper kinetochore assembly and chromosome segregation during mitosis (4). Additional sequence divergence in the histone fold domain is responsible for correct targeting of CENP-A to the centromere (5). Many of the functions of CENP-A are regulated by phosphorylation (6,7). Aurora A-dependent phosphorylation of CENP-A on Ser7 during prophase is required for proper targeting of Aurora B to the inner centromere in prometaphase, proper kinetochore/microtubule attachment and proper alignment of chromosomes during mitosis (6).

染色质结构调控在真核细胞转录和复制的调节中起着重要作用。核小体是染色质的主要构架,由四种核心组蛋白(histones)(H2A,H2B,H3和H4)组成。除了众多的去翻译后组蛋白的修饰调节染色质结构外,细胞也能用组蛋白和类组蛋白互换,这能够直接或间接修饰染色质结构(1)。CENP-A又称作染色质相关蛋白CSE4 (capping-enzyme suppressor 4-p),它是一个本质的组蛋白H3变体,该变体可以取代着丝粒异染色质上的histone H3(2)。CENP-A和规范的组蛋白H3之间最大的差异出现在该蛋白质的氨基端尾,可以结合核小体之间的linker DNA,协助着丝粒的异染色质的正确折叠(3)。CENP-A蛋白的氨基端尾部对其它着丝粒蛋白(CENP-C, hSMC1, hZW10)、正确的着丝点装配和有丝分裂期间染色体的分离也是需要的(4)。组蛋白折叠区域里的附加序列分离是负责CENP-A蛋白正确靶向着丝粒的(5)。CENP-A的多种功能受磷酸化调节(6,7)。CENP-A的Ser7位点在有丝分裂早期的初始阶段的Aurora A依赖的磷酸化对于Aurora B的正确靶向有丝分裂前中期的内部着丝粒、有丝分裂期间正确的着丝点/微管附件以及染色体(6)。

  1. Jin, J. et al. (2005) Trends Biochem Sci 30, 680-7.
  2. Ausió, J. (2006) Brief Funct Genomic Proteomic 5, 228-43.
  3. Heit, R. et al. (2006) Biochem Cell Biol 84, 605-18.
  4. Van Hooser, A.A. et al. (2001) J Cell Sci 114, 3529-42.
  5. Black, B.E. et al. (2004) Nature 430, 578-82.
  6. Kunitoku, N. et al. (2003) Dev Cell 5, 853-64.
  7. Zeitlin, S.G. et al. (2001) J Cell Biol 155, 1147-57.

Application References

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