Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

CENP-A Antibody #2186

cenp-a   centromere-autoantigen-a   centromere-protein-a   h3   histone h3  

No. Size Price
2186S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2186T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
2186 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 17 Rabbit
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

CENP-A Antibody detects endogenous levels of total human CENP-A protein. This antibody does not cross-react with other histone proteins, including Histone H3.

CENP-A Antibody能够检测内源性的人CENP-A总蛋白水平。该抗体不能与其它组蛋白包括Histone H3发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human CENP-A protein. Antibodies are purified by peptide affinity chromatography.

通过合成的与人源CENP-A蛋白相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过多肽亲和层析纯化获得。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using CENP-A Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用CENP-A Antibody (绿色)标记,共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。蓝色= DRAQ5® #4084 (DNA荧光染料)。

Western Blotting

Western Blotting

Western blot analysis of various whole cell lysates using CENP-A Antibody.

使用CENP-A Antibody,免疫印迹(Western blot)分析不同细胞中CENP-A蛋白水平。

Background

Modulation of chromatin structure plays a critical role in the regulation of transcription and replication of the eukaryotic genome. The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure (1). CENP-A, also known as the chromatin-associated protein CSE4 (capping-enzyme suppressor 4-p), is an essential histone H3 variant that replaces canonical histone H3 in centromeric heterochromatin (2). The greatest divergence between CENP-A and canonical histone H3 occurs in the amino-terminal tail of the protein, which binds linker DNA between nucleosomes and facilitates proper folding of centromeric heterochromatin (3). The amino-terminal tail of CENP-A is also required for recruitment of other centromeric proteins (CENP-C, hSMC1, hZW10), proper kinetochore assembly and chromosome segregation during mitosis (4). Additional sequence divergence in the histone fold domain is responsible for correct targeting of CENP-A to the centromere (5). Many of the functions of CENP-A are regulated by phosphorylation (6,7). Aurora A-dependent phosphorylation of CENP-A on Ser7 during prophase is required for proper targeting of Aurora B to the inner centromere in prometaphase, proper kinetochore/microtubule attachment and proper alignment of chromosomes during mitosis (6).

染色质结构调控在真核细胞转录和复制的调节中起着重要作用。核小体是染色质的主要构架,由四种核心组蛋白(histones)(H2A,H2B,H3和H4)组成。除了众多的去翻译后组蛋白的修饰调节染色质结构外,细胞也能用组蛋白和类组蛋白互换,这能够直接或间接修饰染色质结构(1)。CENP-A又称作染色质相关蛋白CSE4 (capping-enzyme suppressor 4-p),它是一个本质的组蛋白H3变体,该变体可以取代着丝粒异染色质上的histone H3(2)。CENP-A和规范的组蛋白H3之间最大的差异出现在该蛋白质的氨基端尾,可以结合核小体之间的linker DNA,协助着丝粒的异染色质的正确折叠(3)。CENP-A蛋白的氨基端尾部对其它着丝粒蛋白(CENP-C, hSMC1, hZW10)、正确的着丝点装配和有丝分裂期间染色体的分离也是需要的(4)。组蛋白折叠区域里的附加序列分离是负责CENP-A蛋白正确靶向着丝粒的(5)。CENP-A的多种功能受磷酸化调节(6,7)。CENP-A的Ser7位点在有丝分裂早期的初始阶段的Aurora A依赖的磷酸化对于Aurora B的正确靶向有丝分裂前中期的内部着丝粒、有丝分裂期间正确的着丝点/微管附件以及染色体(6)。

  1. Jin, J. et al. (2005) Trends Biochem Sci 30, 680-7.
  2. Ausió, J. (2006) Brief Funct Genomic Proteomic 5, 228-43.
  3. Heit, R. et al. (2006) Biochem Cell Biol 84, 605-18.
  4. Van Hooser, A.A. et al. (2001) J Cell Sci 114, 3529-42.
  5. Black, B.E. et al. (2004) Nature 430, 578-82.
  6. Kunitoku, N. et al. (2003) Dev Cell 5, 853-64.
  7. Zeitlin, S.G. et al. (2001) J Cell Biol 155, 1147-57.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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