Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-Rb (Ser608) Antibody #2181

P105-RB   PP105   PP110   RB-1   RB1   Retinoblastoma-associated protein  

No. Size Price
2181S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
2181T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
2181 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Rat,Monkey, Endogenous 110 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Dog,

Specificity / Sensitivity

Phospho-Rb (Ser608) Antibody detects endogenous levels of Rb only when phosphorylated at serine 608.Phospho-Rb (Ser608) Antibody能够检测内源性丝氨酸(608位)磷酸化的Rb蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser608 of human Rb. Antibodies are purified by protein A and peptide affinity chromatography.该多克隆抗体是由合成的人源的针对Rb蛋白丝氨酸(608位)的磷酸化肽段免疫动物,采用蛋白A和多肽亲和层析技术纯化生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells treated with lambda Phosphatase NEB #P0753 (10,000 units/ml, 1 h) or calyculin A #9902 (100 nM, 10 min) using Phospho-Rb (Ser608) Antibody (upper) or Rb (4H1) Mouse mAb #9309 (lower).Western blot方法检测Hela细胞提取物,分为lambda磷酸酶(10,000 units/ml, NEB #P0753)处理1小时组或calyculin A #9902 (100 nM)处理10分钟组,使用的抗体为Phospho-Rb (Ser608) Antibody (上图)和 Rb (4H1) Mouse mAb #9309 (下图)。


The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9). 视网膜母细胞瘤肿瘤抑制蛋白Rb通过控制细胞周期的G1期限制点的进程来调控细胞增殖(1)。Rb具有3个功能特异的结合结构域并能够与转录因子家族的关键调节蛋白E2F、c-Abl酪氨酸激酶和具有保守的LXCXE模体蛋白相互作用(2-4)。CDK介导的细胞周期依赖性磷酸化能够抑制Rb靶向结合并允许细胞周期进程(5)。Rb的失活和随后的细胞周期进程似乎需要伴随周期蛋白E-CDK2磷酸化的cyclin D-CDK4/6初始磷酸化(6)。离体已经观察到不同CDK/cyclin复合物的特异性(6-8),而且周期蛋白D1为第780位丝氨酸在体磷酸化所必需(9)。

  1. Sherr, C.J. (1996) Science 274, 1672-7.
  2. Nevins, J.R. (1992) Science 258, 424-9.
  3. Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.
  4. Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.
  5. Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.
  6. Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
  7. Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.
  8. Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.
  9. Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :